陆地棉杯状叶突变体基因的SSR分子标记定位  

Mapping of a Cup Leaf Mutant Gene in Gossypium hirsutum by SSR Markers

作　　者：陈旭升[1] 赵龙飞 赵亮[1] 狄佳春[1] Chen Xusheng;Zhao Longfei;Zhao Liang;Di Jiachun(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Canola Research at the Lower Reach of the Yangtze River Plain,Ministry of Agriculture and Rural Affairs,Nanjing 210014)

机构地区：[1]江苏省农业科学院经济作物研究所/农业农村部长江下游棉花与油菜重点实验室,南京210014

出　　处：《中国农学通报》2021年第31期1-4,共4页Chinese Agricultural Science Bulletin

基　　金：转基因生物新品种培育科技重大专项项目“转基因特色专用棉新品种培育”(2020ZX08005-005)。

摘　　要：为研究陆地棉杯状叶变异的分子遗传规律,以自主鉴定的陆地棉绿色杯状叶突变体‘R169’为试验材料,通过与海岛棉品种‘胜利1号’配制海陆杂交组合进行遗传分析,结果显示,杯状叶突变体为单位点质量性状突变体,符合由一对隐性基因控制的孟德尔分离规律。而后利用棉花单位点质量基因在染色体的快速定位方法对该突变体基因(cup)进行染色体定位,对12个特定样本组成的小群体分析显示,引物NAU3480、BNL1066与目的基因cup连锁。由于引物NAU3480、BNL1066均位于棉花第11染色体,据此推断cup基因位于第11染色体。而后合成位于该染色体的110对SSR引物,通过对双亲DNA的PCR扩增得到26对多态性引物。用这些引物检测F2代群体每个单株的标记基因型,然后通过软件进行遗传连锁分析,结果表明共有19对引物与基因cup连锁,它们分别是引物NAU5192、NAU3621、NAU2118、NAU1162、NAU3409、NAU2998、NAU3657、NAU1148、NAU3234、NAU2651、NAU2599、NAU2661、NAU1014、NAU3622、BNL1408、NAU4086、BNL1066、NAU3480、NAU5505。基因cup位于SSR分子标记BNL1066与NAU3480之间,遗传距离分别为9.3 cM和1.6 cM。To study the molecular genetic law of a cup leaf mutant in Gossypium hirsutum, a self-identified cotton cup leaf mutant‘R169’was used as the experimental material, the sea-land hybrid combination was conducted with the island cotton variety‘ Shengli 1’. The genetic analysis showed that the cup leaf mutant was a mono-locus quality character mutant, controlled by a pair of recessive genes and fit the Mendel pattern.Then the method of rapid mapping of the mono-locus quality character gene on chromosome was used to locate the mutant cup gene. The results by checking 12 specific samples showed that the primers NAU3480 and BNL1066 were linked to the cup gene. As the primers NAU3480 and BNL1066 were located on the 11 th chromosome of cotton, it was inferred that the cup gene was located on the 11 thchromosome. Then 110 pairs of primers on the chromosome were synthesized and 26 pairs of polymorphic primers were obtained by PCR parental DNA. These primers were used to detect the marked genotype of each individual plant in F2 population and then genetic linkage analysis was performed by software. The results showed that 19 pairs of primers were linked to the gene cup, which were NAU5192, NAU3621, NAU2118, NAU1162, NAU3409,NAU2998, NAU3657, NAU1148, NAU3234, NAU2651, NAU2599, NAU2661, NAU1014, NAU3622,BNL1408, NAU4086, BNL1066, NAU3480, NAU5505. Gene cup was located between BNL1066 and NAU3480, and the genetic distance was 9.3 c M and 1.6 c M, respectively.

关 键 词：陆地棉 杯状叶突变体 SSR分子标记 单位点质量性状基因 染色体定位 

分 类 号：S330[农业科学—作物遗传育种]