赖氨匹林通过RAS/RAF/ERK信号通路调控子宫内膜癌细胞增殖、凋亡的研究机制  

Aspisol regulates the proliferation and apoptosis of endometrial cancer cells through RAS/RAF/ERK signaling pathway

作　　者：李娜[1] 宋泓[1] 王茜 LI Na;SONG Hong;WANG Qian(Chenzhou First People’s Hospital Central Hospital,Chenzhou,Hunan 423000,China)

机构地区：[1]郴州市第一人民医院中心医院,湖南郴州423000

出　　处：《中国优生与遗传杂志》2021年第6期781-785,共5页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究赖氨匹林(Aspisol)对子宫内膜癌细胞增殖、凋亡的影响及其对RAS/RAF/ERK信号通路的调控作用。方法采用赖氨匹林(5μmol/L、10μmol/L、15μmol/L)作用于子宫内膜癌ZJB-ENC1细胞,MTT法检测赖氨匹林对子宫内膜癌ZJB-ENC1细胞增殖的影响;流式细胞术检测赖氨匹林对细胞凋亡的影响。采用RAS/RAF/ERK信号通路激活剂RASAL1处理子宫内膜癌细胞并加入赖氨匹林(10μmol/L)共同作用(RASAL1+Aspisol组),检测细胞增殖及凋亡能力变化。荧光定量PCR与蛋白免疫印迹(Western blot)分别检测赖氨匹林作用子宫内膜癌细胞后Cyclin D1、Bcl-2、Ras、Raf、p-Erk1/2 mRNA及蛋白的表达。结果MTT检测发现赖氨匹林对ZJB-ENC1细胞生长具有明显抑制作用,且与赖氨匹林使用浓度呈正相关;流式细胞术检测发现随着赖氨匹林浓度的增高细胞凋亡率明显升高;qRT-PCR与Western blot检测发现,与control组相比,赖氨匹林可明显抑制Cyclin D1、Bcl-2、Ras、Raf、p-Erk1/2表达,与Aspisol组相比,RASAL1+Aspisol组Cyclin D1、Bcl-2、Ras、Raf、p-Erk1/2表达均明显降低。结论赖氨匹林可通过抑制RAS/RAF/ERK信号通路相关蛋白表达而抑制其信号通路激活进程进而诱导子宫内膜癌细胞凋亡并抑制其增殖。Objective To study the effect of aspisol on proliferation and apoptosis of endometrial cancer cells and its regulation on RAS/RAF/ERK signaling pathway. Methods Aspisol(5 μmol/L, 10 μmol/L, 15 μmol/L) was applied to endometrial cancer ZJB-ENC1 cells, and MTT assay was used to detect lysine against endometrial cancer ZJB-ENC1 effect of cell proliferation. Flow cytometry was used to detect the effect of lysine on apoptosis. Endometrial cancer cells were treated with RAS/RAF/ERK signaling pathway activator RASAL1 and lysamine(10 μmol/L) was added(RASAL1+Aspisol group) to detect changes in cell proliferation and apoptosis. Real-time PCR and Western blot were used to detect the expression of Cyclin D1, Bcl-2, Ras, Raf and p-Erk1/2 mRNA and protein, respectively, after aspisol was used to treat endometrial cancer cells.Results MTT assay showed that aspisol had a significant inhibitory effect on the growth of ZJB-ENC1 cells and was positively correlated with the concentration of aspisol. Flow cytometry showed that the apoptosis rate increased significantly with the increase of aspisol concentration. qRT-PCR and Western blot analysis showed that aspisol could significantly inhibit the expression of Cyclin D1, Bcl-2, Ras, Raf and p-Erk1/2 compared with the control group. Compared with the Aspisol group, the RASAL1+Aspisol group. The expression of Cyclin D1, Bcl-2, Ras, Raf and p-Erk1/2 was significantly decreased. Conclution aspisol can inhibit the proliferation of endometrial cancer cells by inhibiting the expression of RAS/RAF/ERK signaling pathway-associated proteins.

关 键 词：赖氨匹林 RAS/RAF/ERK信号通路 增殖 凋亡 

分 类 号：R965[医药卫生—药理学]