Inactivated Pseudomonas PE(△Ⅲ)exotoxin fused to neutralizing epitopes of PEDV S proteins produces a specific immune response in mice  

作　　者：Leqiang Sun Yajie Tang Keji Yan Huanchun Chen Huawei Zhang 

机构地区：[1]State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,Hubei,China [2]Laboratory of Animal Virology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,Hubei,China [3]Henan Joint International Research Laboratory of Veterinary Biologies Research and Application,Anyang Institute of Technology,Anyang 455000,Henan,China [4]College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China

出　　处：《Animal Diseases》2021年第3期205-211,共7页动物疾病（英文）

基　　金：This research was funded by the National Natural Science Foundation of China(31900746 and 31802170);Postdoctoral Science Foundation(2019M660182).

摘　　要：Porcine epidemic diarrhea(PED)caused by the porcine epidemic diarrhea virus(PEDV),is a severe infectious and devastating swine disease that leads to serious economic losses in the swine industry worldwide.An increased number of PED cases caused by variant PEDV have been reported in many countries since 2010.S protein is the main immunogenic protein containing some B-cell epitopes that can induce neutralizing antibodies of PEDV.In this study,the construction,expression and purification of Pseudomonas aeruginosa exotoxin A(PE)without domain Ⅲ(PE△Ⅲ)as a vector was performed for the delivery of PEDV S-A or S-B.PE(△Ⅲ)PEDV S-A and PE(△Ⅲ)PEDV S-B recombinant proteins were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis.The immunogenicity of PEDV S-A and PEDV S-B subunit vaccines were evaluated in mice.The results showed that PEDV-S-B vaccine could not only induce specific humoral and Th1 type-dominant cellular immune responses,but also stimulate PEDV-specific mucosal immune responses in mice.PEDV-S-B subunit vaccine is a novel candidate mucosal vaccine against PEDV infection.

关 键 词：Porcine epidemic diarrhea virus S protein Neutralizing antibody Th1-type immune response 

分 类 号：S85[农业科学—兽医学]