柯萨奇病毒A9型山东地方株的进化遗传学特征  

Evolutionary Genetics of Coxsackievirus A9 in Shandong Province,China

作　　者：黄蓉蓉 林小娟[2] 李漫时[2] 刘尧[2] 熊萍[2] 赵晨旭 陶泽新[2] 徐爱强 HUANG Rongrong;LIN Xiaojuan;LI Manshi;LIU Yao;XIONG Ping;ZHAO Chenxu;TAO Zexin;XU Aiqiang(School of Public Health,Cheeloo College of Medicine,Shandong University,Jinan 250012,China;Shandong Provincial Center for Disease Control and Prevention Key Laboratory of Infectious Disease Prevention and Control in Shandong Province,Jinan 250014,China)

机构地区：[1]山东大学齐鲁医学院公共卫生学院,济南250012 [2]山东省疾病预防控制中心山东省传染病预防控制重点实验室,济南250014

出　　处：《病毒学报》2021年第6期1333-1340,共8页Chinese Journal of Virology

基　　金：泰山学者工程专项经费资助(项目号:ts.201511105),研究方向:病毒性及疫苗可预防传染病防控;泰山学者青年专家计划项目(项目号:tsqn202103187),研究方向:基于同一健康理念和下一代测序技术新型病毒的发现、溯源和进化动态研究;国家自然科学基金(项目号:81302481),题目:基于环境污水监测的人类肠道病毒区域性流行遗传变异与重组规律的研究。

摘　　要：柯萨奇病毒A9型(Coxsackievirus A9,CVA9)是常见的人类肠道病毒血清型,其感染可引起无菌性脑膜炎、脑炎等疾病。为探索其进化遗传学特征,本研究对山东省1991-2018年CVA9分离株的VP1完整编码区进行了序列测定,并与GenBank中获得的全球序列一并进行系统发生学和进化遗传学分析。结果显示全球CVA9可分为I-XII 12个基因型,优势基因型为VII,包括山东株在内的所有中国分离株均属于该基因型。进化遗传学研究显示,CVA9 VP1区序列的每年每个碱基的平均进化速率约为6.25×10^(-3),共同祖先起源于1919年。CVA9 VP1区的基因多样性在2003年以前无明显变化,2003年以后出现明显波动。本研究为我们了解CVA9的流行趋势和遗传变异提供了数据,对CVA9相关疾病的防控和诊断试剂的研发具有重要的现实意义。Coxsackievirus A9(CVA9)is one of the most common serotypes of human enteroviruses. Infection with CVA9 leads to aseptic meningitis,encephalitis,etc. We wished to understand the evolutionary genetics of CVA9. Sequencing of major capsid protein VP1 was done on CVA9 isolates collected in Shandong Province,China,from 1991 to 2018. Their phylogenetic characterization and evolutionary dynamics were analyzed together with global sequences from the GenBank database. We discovered that global CVA9 could be divided into 12 genotypes(I–XII),with VII being predominant. All Chinese isolates(including those from Shandong)belonged to this genotype. The average evolutionary rate of VP1 region of CVA9 was 6.25×10^(-3) substitutions/site/year,and the common ancestor originated in 1919. The genetic diversity of the VP1 region did not vary significantly before 2003,but fluctuated significantly after 2003. Our results aid understanding of the epidemic trend and genetic variation of CVA9. They also have practical importance for the prevention and control of CVA9-related diseases as well as development of diagnostic reagents.

关 键 词：柯萨奇病毒A9型(CVA9) 系统发生学 进化遗传学 无菌性脑膜炎(AM) 

分 类 号：R373.2[医药卫生—病原生物学]