白灵侧耳野生种质群体生长发育相关性状评价  

Evaluation of the Growth and Development Traits for Wild Germplasm of Pleurotus tuoliensis

作　　者：杨雅舒 汪颖 高巍[1] YANG Ya-shu;WANG Ying;GAO Wei(Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Key Laboratory of Microbial Resources,Ministry of Agriculture and Rural Affairs,Beijing 100081;Inner Mongolia University of Science and Technology Baotou Teachers’College,Baotou 014030)

机构地区：[1]中国农业科学院农业资源与农业区划研究所农业农村部微生物收集与保藏重点实验室,北京100081 [2]内蒙古科技大学包头师范学院,包头014030

出　　处：《生物技术通报》2021年第11期101-108,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31701979);国家现代农业产业技术体系资助(CARS-20)。

摘　　要：通过对野生种质群体生长发育性状的表型鉴定和分析,揭示我国特有种类白灵侧耳种质资源的多样性和可利用性。从国家标准菌株库的库藏中随机选择了100个白灵侧耳野生菌株,对其生长发育性状,即PDA培养基平板中的菌丝生长速度、棉籽壳培养料试管中的菌丝长速及原基形成时间等3个性状进行了表型测定和分析。根据性状数据的统计分析发现3个性状均为多基因控制的数量性状,且性状间不具有显著的相关性;菌丝长速性状具有较高的广义遗传力,说明性状的遗传变异主要由基因型决定。来源于不同地区的菌株原基形成所需的时间存在显著性差异,发现来自于托里县的菌株原基形成所需的时间最短,而来自于青河县的菌株原基形成时间最长,推测可能由不同来源地菌株的进化机制不同造成。研究结果为发掘白灵侧耳育种的可利用种质提供了科学依据,为将优异种质资源转化为可利用的亲本材料提供理论基础。The phenotypic identification and analysis of growth and development traits for the wild germplasm of Pleurotus tuoliensis were conducted,thereby the diversity and the utilizability of the germplasm from the special P.tuoliensis were explored.A total of 100 wild strains were randomly selected from the collection of China Center for Mushroom Spawn Standards and Control(CCMSSC).The mycelial growth rate on PDA plates and that in substrate tubes of cottonseed husk as culture material,as well as the formation time of primordia were tested and analyzed.According to the results of statistics,the three tested traits were all quantitative traits controlled by multiple loci,and there was no significant correlation among the three tested traits.The broad-sense heritability of the mycelial growth rates was high,indicating that the phenotypic variation of traits was mainly determined by genotypes although influenced by environments.Primordium formation time of strain group from different geographical origin varied significantly,the shortest for the strain from Tuoli,and the longest for the strains from Qinghe,suggesting that it resulted from the different evolution mechanism for different-origin strains.The findings of this study provides scientific basis for exploring useful germplasm in breeding P.tuoliensis and theoretical foundation for converting excellent germplasm into useful parent material.

关 键 词：白灵侧耳 野生种质 菌丝长速 原基形成 表型评价 

分 类 号：S646[农业科学—蔬菜学]