链格孢菌产细交链孢菌酮酸条件的优化研究  

Study on Optimization of Conditions for Production of Tenuazonic Acid in Alternaria alternata

作　　者：许雁祥 唐敏[1,2] 李云仙 杨发忠 Xu Yanxiang;Tang Min;Li Yunxian;Yang Fazhong(Key Laboratory of Conservation and Utilization of Forest Resources in Southwest China,Kunming Yunnan 650233,China;College of Chemical Engineering,Southwest Forestry University,Kunming Yunnan 650233,China)

机构地区：[1]西南山地森林资源保育与利用教育部重点实验室,云南昆明650233 [2]西南林业大学化学工程学院,云南昆明650233

出　　处：《西南林业大学学报（自然科学）》2021年第6期109-115,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：西南林业大学校级科研专项项目(111908)资助;云南省教育厅科学研究基金项目(2020J0399)资助;西南山地森林资源保育与利用教育部重点实验室开放项目(KLESWFU-201806)资助;国家自然科学基金项目(31560517)资助;国家自然科学基金项目(31260504)资助;云南省中青年学术与技术带头人培养项目(2017HB031)资助。

摘　　要：鉴于链格孢霉毒素细交链孢菌酮酸(TeA)对防治月季长管蚜有明显效果,为了进一步开发利用TeA,对1株链格孢菌(编号5390)产TeA的条件进行研究,通过单因素试验的方法,研究了不同培养基、不同培养温度、不同培养时间及不同光照条件对TeA含量的影响,在此基础上用响应面对链格孢菌产毒条件进行优化。结果表明产TeA的最佳条件为:理查培养基,黑暗培养,温度30℃,培养时间29 d。在此条件下进行试验验证,TeA含量为14.99μg/g,与模型预测接近。对链格孢属真菌产TeA的培养条件进行筛选,探索真菌培养方式获得链格孢菌毒素TeA的方法,将有利于TeA的进一步开发利用。Tenuazonic acid(TeA)can be used to control aphid,which has obvious effect.In order to further develop and utilize TeA,the conditions of TeA production by Alternaria alternata(No.:5390)were studied.The effects of different media,temperature,time and illumination on TeA content were studied by single factor experiment.On this basis,response surface was used to optimize the production conditions of A.alternata.The results show that the optimal conditions were Richards medium,dark culture,temperature 30℃for 29 days.Under these conditions,the TeA content was 14.99μg/g,which was close to the model prediction.Screening the culture conditions of A.alternata producing TeA and exploring the method of obtaining A.alternata toxin TeA by fungus culture will be beneficial to the further development and utilization of TeA.

关 键 词：链格孢菌 产毒条件 细交链孢菌酮酸 响应面法 高效液相色谱法 

分 类 号：S718.81[农业科学—林学]