硝酸镧对脐橙叶片2种膜透性物质含量及PPO活性的影响  

Effect of Lanthanum Nitrate on the Content of Two Osmoregulatory Substances and PPO Activity in Navel Orange Leaves

作　　者：张小丽 陈璐 高柱[1] 卢玉鹏 王小玲[1] ZHANG Xiao-li;CHEN Lu;GAO Zhu(Institute of Biological Resources,Jiangxi Academy of Sciences,Nanchang,Jiangxi 330096;Jinggangshan Institute of Biotechnology,Ji’an,Jiangxi 343016)

机构地区：[1]江西省科学院生物资源研究所,江西南昌330096 [2]井冈山生物技术研究院,江西吉安343016

出　　处：《安徽农业科学》2021年第23期43-45,66,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31760551)。

摘　　要：以2年生纽荷尔脐橙嫁接苗为材料,通过喷施50、150、300 mg/L的硝酸镧[La(NO_(3))_(3)]溶液,清水作为对照(CK),测定喷施前(0 h)和喷施后(2、4、8、12、24 h)脐橙叶片中2种膜透性物质过氧化氢(H_(2)O_(2))与丙二醛(MDA)含量及多酚氧化酶(PPO)活性的变化特性,阐明硝酸镧对脐橙叶片H_(2)O_(2)和MDA含量及PPO活性的影响。结果表明,硝酸镧处理后,脐橙叶片H_(2)O_(2)含量和PPO活性呈"先升高,后降低"的变化趋势。150和300 mg/L硝酸镧处理不仅明显降低了脐橙叶片H_(2)O_(2)含量增加速度,延缓了H_(2)O_(2)含量峰值出现时间,而且明显提高了脐橙叶片PPO活性,其中150 mg/L的效果较好。另外,50 mg/L硝酸镧处理后脐橙叶片MDA含量峰值与CK出现的时间相同,处理后24 h,脐橙叶片MDA含量比CK降低了30%,而150和300 mg/L处理则明显促进了脐橙叶片MDA含量的积累。因此,硝酸镧对脐橙叶片中H_(2)O_(2)、MDA和PPO的影响符合"低促高抑"的效应,适宜的处理浓度为50~150 mg/L。The 2-year-old‘Newhall’navel oranges grafted seedlings were sprayed with 50,150,300 mg/L La(NO_(3))_(3)solution,and water was used as control(CK).The content of hydrogen peroxide(H_(2)O_(2)),malondialdehyde(MDA)and the activity of polyphenol oxidase(PPO)in leaves were measured at different times(0,2,4,8,12 and 24 h),to clarify the effect of lanthanum nitrate on the H_(2)O_(2)and MDA content and PPO activity of navel orange leaves.The results showed that after be treated with La(NO_(3))_(3),the H_(2)O_(2)content and PPO activity increased first and then decreased.The treatments of 150 and 300 mg/L not only significantly reduced the increase rate and delayed the peak time of H_(2)O_(2)content,but also significantly improved the PPO activity,of which 150 mg/L had a better effect.After lanthanum nitrate treatment with 50 mg/L,the peak MDA content appeared at the same time as CK.24 h after treatment,the MDA content decreased by 30%compared with CK,while 150 and 300 mg/L treatment significantly promoted the accumulation of MDA content.Therefore,the effect of lanthanum nitrate on H_(2)O_(2),MDA and PPO in navel orange leaves conformed to the effect of"low promotion and high inhibition",and the appropriate treatment concentration ranged from 50 to 150 mg/L.

关 键 词：硝酸镧 纽荷尔脐橙 过氧化氢 丙二醛 多酚氧化酶 

分 类 号：S666[农业科学—果树学]