上海市造血干细胞捐献志愿者口腔拭子样本的分型成功率及其影响因素  

Success Rate of Typing of Buccal Swab Samples from Hematopoietic Stem Cell Donation Volunteers in Shanghai and Its Influencing Factors

作　　者：朱臻逸 杜克明 廖宽镇 刘宝松[1,2] 张钢 吕军[1,2] ZHU Zhenyi(School of Public Health,Fudan University,Shanghai,200032,China)

机构地区：[1]复旦大学公共卫生学院,上海200032 [2]复旦大学中国残疾问题研究中心,上海200032 [3]上海荻硕贝肯医学检验所有限公司,上海201318 [4]上海市红十字会,上海200040

出　　处：《医学与社会》2021年第12期1-4,共4页Medicine and Society

基　　金：上海市红十字会课题,编号为WAF201037;国家社会科学基金资助重大项目,编号为17ZDA078;国家自然科学基金资助项目,编号为71774030。

摘　　要：目的:了解上海市造血干细胞捐献志愿者的口腔拭子样本分型成功率情况,分析其影响因素,为提高口腔拭子样本分型成功率提供参考。方法:利用2015-2018年上海市口腔拭子入库志愿者的8104条口腔拭子样本检测结果和造血干细胞资料库信息,对不同年份、不同采样方式获得的样本分型成功率进行卡方分析,并对口腔拭子样本分型结果的影响因素进行回归分析。结果:2015-2018年,造血干细胞入库志愿者的口腔拭子样本分型成功率逐年上升(P<0.05),2018年的口腔拭子样本与静脉血样本在分型成功率上无差异(P>0.05);logistic回归分析显示,样本分型的有效性在户籍、献血经历与采集检测的时间间隔上差异有统计学意义(P<0.05),送检时间间隔较短、上海户籍以及没有献血经历的志愿者口腔拭子样本具有更高的分型成功率。结论:口腔拭子样本的分型成功率较为理想;志愿者户籍、献血经历和采集检测时间间隔是影响口腔拭子样本分型结果的主要因素,需要优化关键环节来保障口腔拭子技术的有效应用。Objective:To investigate the success rate of buccal swab typing from hematopoietic stem cell donation volunteers in Shanghai,and to analyze the influencing factors,so as to provide reference for improving the success rate of buccal swab sample typing.Methods:The detection results and hematopoietic stem cell database information of 8104 buccal swab samples from volunteers in Shanghai from 2015 to 2018 were used.Chi-square analysis was used to analyze the success rate of sample typing obtained in different years and different sampling methods,and regression analysis was carried out on the factors affecting the typing results of buccal swab samples.Results:From 2015 to 2018,the success rate of buccal swab typing of volunteers with hematopoietic stem cells increased year by year(P<0.05).There was no significant difference in typing success rate between buccal swab samples and venous blood samples in 2018(P>0.05).Logistic regression analysis showed that the validity of sample typing was significantly different in household registration,blood donation experience and time interval of collection and testing(P<0.05).The buccal swab samples of volunteers with shorter time interval,Shanghai household registration and no blood donation experience had higher success rate of typing.Conclusion:The typing success rate of buccal swab samples is satisfactory.The household registration of volunteers,the experience of blood donation and the time interval of collection and testing are the main factors that affect the results of buccal swab subsample typing,and these key links need to be optimized to ensure the effective application of buccal swab technology.

关 键 词：造血干细胞 口腔拭子 DNA分型 上海 

分 类 号：R193[医药卫生—卫生事业管理]