基于CRISPR/Cas的检测技术发展及其在植物病原物检测中的应用  

Development of CRISPR/Cas-based detection method and its application in plant pathogens

作　　者：雷荣[1] 费鑫宇 李明福[1] 吴品珊[1] 王新一 Lei Rong;Fei Xinyu;Li Mingfu;Wu Pinshan;Wang Xinyi(Chinese Academy of Inspection and Quarantine,Beijing 100176,China;College of Land and Environment of Shenyang Agricultural University;School of Life Science and Biotechnology,Dalian University)

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]沈阳农业大学土地与环境学院 [3]大连大学生命科学与技术学院

出　　处：《植物检疫》2021年第6期1-10,共10页Plant Quarantine

基　　金：中国检验检疫科学研究院基本科研业务费项目(2020JK048);辽宁省科学研究经费重点攻关项目(LSNZD201901);辽宁省科学技术基金项目(2019-MS-270)。

摘　　要：CRISPR/Cas系统是一种高效、实用的基因编辑工具。该系统与多种核酸扩增技术结合,已被开发出形式多样、灵敏度高、特异性好的病原物核酸检测方法。本文简要介绍了CRISPR/Cas的分类和作用原理,重点综述了CRISPR/Cas系统在各种病原微生物检测中的应用,讨论了CRISPR/Cas在植物病原物的现场快速检测方面的最近进展,并对CRISPR/Cas应用于植物病原物的检测前景进行了展望。CRISPR/Cas system is a promising new genomic sensing method,which has been combined with many nucleic acid amplification techniques and different read-out methods,to produce a variety of diagnosis tools for pathogens with considerable sensitivity and specificity.This review covers a short introduction to CRISPR/Cas systems,the types of effector proteins and the recent developments of CRISPR sensing techniques for pathogens detection.In particular,the application of CRISPR/Cas in the rapid on-site detection of pathogens is comprehensively reviewed.Finally,the challenges and future research perspectives on plant pathogens detection were described.

关 键 词：CRISPR/Cas系统 基因检测工具 核酸检测 植物病原物 

分 类 号：Q812[生物学—生物工程]