CdTe和CdTe/CdS量子点与叶酸分子荧光相互作用  

Fluorescence Interaction Between Folate Molecules and CdTe and CdTe/CdS Quantum Dots

作　　者：李光旻 刘淏洋 杨洋 刘洪飞 费学宁 刘伟 LI Guang-min;LIU Hao-yang;YANG Yang;LIU Hong-fei;FEI Xue-ning;LIU Wei(School of Science, Tianjin Chengjian University, Tianjin 300384, China;Zhongqi Zhongxin(Tianjin) Industrial Design Co., Ltd., Tianjin 300384, China)

机构地区：[1]天津城建大学理学院,天津300384 [2]中企众鑫(天津)工业设计有限公司,天津300384

出　　处：《发光学报》2021年第12期1944-1950,共7页Chinese Journal of Luminescence

基　　金：国家自然科学基金(51578360,51778398);天津市高等学校基本科研业务资助项目(170401017);天津市技术创新引导专项(基金)(21YDTPJC00970)资助项目。

摘　　要：制备了水溶性半胱氨酸修饰的CdTe和CdTe/CdS核壳结构量子点,实现了对叶酸分子的特异性荧光标记,可作为具有应用潜力的叶酸浓度快速测定荧光探针。实验结果表明,叶酸分子可以使CdTe量子点发生明显的荧光猝灭,而在CdTe/CdS核壳结构量子点中,没有观察到这种猝灭行为。本文采用Stern-Volmer方程对荧光猝灭机制进行了分析,研究表明该猝灭机制是静态荧光猝灭。CdTe量子点在与叶酸分子作用过程中,荧光光谱发射峰发生了约35 nm的蓝移,热力学计算表明,量子点可能与叶酸分子通过静电作用形成了络合结构。Water soluble cysteine modified CdTe and CdTe/CdS core-shell quantum dots were prepared,which can realize the specific fluorescent labeling of folate molecules,and function as a fluorescent probe for detecting the concentration of folate.The experimental results showed that folate can cause obvious fluorescence quenching of CdTe quantum dots,but this quenching behavior was not observed in CdTe/CdS core-shell system.The fluorescence quenching mechanism was analyzed by Stern-Volmer equation,and the research showed that it was a static fluorescence quenching mechanism.The fluorescence emission spectrum had a blue shift of about 35 nm in the interaction between CdTe quantum dots and folate molecules.Thermodynamic calculation showed that a complex structure may be formed through electrostatic interaction between the quantum dots and folate molecules.

关 键 词：荧光 量子点 叶酸 猝灭 CDTE CdTe/CdS核壳结构 

分 类 号：O482.31[理学—固体物理]