251份欧洲冬小麦成株期抗条锈病鉴定及抗条锈病基因检测  

Identification of Resistance to Stripe Rust and Detection of Stripe Rust Resistance Genes in 251 European Winter Wheats at Adult Stage

作　　者：翟雪婷 赵池铭 汪军成 姚立蓉[1,4] 司二静 王化俊 孟亚雄[1,4] 岳维云 尚勋武[4] 李葆春 ZHAI Xueting;ZHAO Chiming;WANG Juncheng;YAO Lirong;SI Erjing;WANG Huajun;MENG Yaxiong;YUE Weiyun;SHANG Xunwu;LI Baochun(Gansu Provincial Key Laboratory of Aridland Crop Science,Gansu Provincial Key Laboratory of Crop Improvement&Germplasm Enhancener,Lanzhou 730070,China;College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;Lintao Agricultural School,Dingxi City,Dingxi 730500,China;College of Agriculture,Gansu Agricultural University,Lanzhou 730070,China;Tianshui Agricultural Science Research Institute,Tianshui 741000,China)

机构地区：[1]甘肃省干旱生境作物学重点实验室,甘肃省作物遗传改良与种质创新实验室,甘肃兰州730070 [2]甘肃农业大学生命科学技术学院,甘肃兰州730070 [3]定西市临洮农业学校,甘肃定西730500 [4]甘肃农业大学农学院,甘肃兰州730070 [5]天水市农业科学研究所,甘肃天水741000

出　　处：《山西农业科学》2021年第12期1444-1452,共9页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31860377);财政部和农业农村部:国家现代农业产业技术体系(CARS-05-04B-2);甘肃省教育厅产业支撑计划项目(2021CYZC-12);甘肃省教育厅高校科研项目(2018D-14);甘肃省科技重大专项(17ZD2NA016);甘肃省干旱生境作物学重点实验室开放基金项目(GSCS-2016-03);甘肃省高等学校青年博士基金项目(2021QB-029)。

摘　　要：研究旨在明确251份欧洲20世纪育成的冬小麦代表性品种对中国条锈菌的抗性水平以及所含的抗病基因,为拓宽抗源的遗传基础、培育抗条锈病小麦新品种以及抗源的合理利用提供依据。利用甘肃天水地区良好的自然诱发条件,连续2 a对251份欧洲冬小麦品种进行田间成株期抗病性鉴定,并通过分子标记对4个已知的抗条锈病基因(Yr10、Yr15、Yr18、Yr26)进行检测,以确定所筛选的抗病材料是否携带已知的抗病基因。结果表明,通过田间成株期鉴定,发现抗病的材料有141份,其中表现为免疫的45份、高抗的72份、中抗的24份,表现为中感和高感的材料有110份。分子标记检测结果表明,携带Yr10、Yr15、Yr18基因的品种分别有146、28、27份,分别占供试小麦材料的58.17%、11.16%、10.76%,Yr26分子标记在试验材料中未检测到;89份材料未检测到携带上述已知抗条锈病基因,其中41份具条锈病抗性,推测可能含有其他已知或未知的抗病基因。研究初步明确了部分欧洲材料的抗性水平和抗条锈病基因特征,筛选出141份具有不同抗性水平的抗源材料,其中100份材料含已知的抗条锈病基因,其余41份可能含有新抗源。The purpose of this study was to determine the resistance level and resistance genes of 251 representative wheat varieties bred in Europe in the 20th century to Chinese stripe rust,and to provide basis for broadening the genetic basis of resistance sources,breeding new wheat varieties resistant to stripe rust and rational utilization of resistance sources.According to the favorable natural inducing conditions in Tianshui area of Gansu province,251 European varieties were identified for disease resistance at adult stage in the field for two years.Four known stripe rust resistance genes(Yr10,Yr15,Yr18 and Yr26)were detected by molecular markers to determine whether the selected resistant materials carried known resistance genes.The result showed that 141 materials were resistant to disease,among which 45 were immune,72 were highly resistant,24 were moderately resistant,and 110 were moderately susceptible or highly susceptible.The result of molecular marker detection showed that 146,28 and 27 cultivars carried Yr10,Yr15 and Yr18 genes,respectively,accounting for 58.17%,11.16%and 10.76%of the tested wheat materials,respectively,the gene of Yr26 was not been found.89 materials were not detected to carry the above known rust resistance genes,and 41 of them were resistant to stripe rust,presumably containing other known or unknown resistance genes.This study preliminary defined the resistance level and gene characteristics of stripe rust resistance of some European materials,and found 141 test materials with different disease resistant levels,of which 100 detected known disease resistance genes and 41 may contain new resistance sources.

关 键 词：小麦 条锈病 成株期 抗病种质 抗条锈病基因检测 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]