草菇过氧化氢酶基因(VvCAT1)异源表达及耐温度胁迫功能分析  

Expression of catalase gene(VvCAT1) from Volvariella volvacea in Escherichia coli and its temperature tolerance

作　　者：杨焕玲[1] 常婷婷 赵妍[1] 游华芳 查磊[1] 李正鹏[1] 仝宗军 陈明杰[1] YANG Huanling;CHANG Tingting;ZHAO Yan;YOU Huafang;ZHA Lei;LI Zhengpeng;TONG Zongjun;CHEN Mingjie(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]上海市农业科学院食用菌研究所,上海201403

出　　处：《微生物学通报》2021年第11期4054-4060,共7页Microbiology China

基　　金：上海市科技兴农项目(2020-02-08-00-12-F01479)。

摘　　要：【背景】过氧化氢酶(Catalase,CAT)参与真菌的生长发育,逆境胁迫时保护真菌免受氧化损伤。【目的】实现草菇过氧化氢酶基因(VvCAT1)的异源表达,分析VvCAT1耐温度胁迫的功能。【方法】克隆VvCAT1,构建过表达载体pBAR GPE1/VvCAT1,转化到大肠杆菌(Escherichia coli)菌株Stbl3中,异源表达草菇过氧化氢酶。测定温度胁迫后重组菌(pBARGPE1/VvCAT1/Stbl3)与对照菌(pBARGPE1/Stbl3)的过氧化氢酶活性和生长情况,验证VvCAT1的功能。【结果】重组菌的CAT酶活性显著提高,生长情况显著优于对照菌。【结论】VvCAT1的导入及表达显著提高了大肠杆菌Stbl3的耐温度胁迫功能。[Background] Catalase(CAT) is involved in the growth and development of fungi and protects them from oxidative damage in the event of stress. [Objective] The VvCAT1 gene from Volvariella volvacea was expressed in Escherichia coli Stbl3 and its temperature tolerance was characterized.[Methods] VvCAT1 was cloned to construct a recombinant plasmid pBAR GPE1/VvCAT1 which was then transformed into Stbl3 to yield a recombinant strain expressing catalase. Catalase activity and growth of the recombinant strain(pBAR GPE1/VvCAT1/Stbl3) and the control strain(pBAR GPE1/Stbl3) were measured to verify the temperature tolerance. [Results] The recombinant strain exhibited stronger heat and cold tolerance than the control with increased catalase activity and better growth. [Conclusion] The transgenosis and expression of VvCAT1 significantly improved the heat and cold tolerance of E. coli Stbl3.

关 键 词：草菇 过氧化氢酶 异源表达 耐温性 

分 类 号：S646.13[农业科学—蔬菜学]