担子菌门总革菌科中国新记录种紫黑点壳菌  

Punctularia atropurpurascens(Punctulariaceae, Basidiomycota): a new record to China

作　　者：彭子嘉 余安 骆泽煜 刘小勇[3] 陈万福 余仲东[1] PENG Zijia;YU An;LUO Zeyu;LIU Xiaoyong;CHEN Wanfu;YU Zhongdong(College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China;College of Forestry,Central South University of Forestry and Technology,Changsha,Hunan 410004,China;Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]中南林业科技大学林学院,湖南长沙410004 [3]中国科学院微生物研究所,北京100101

出　　处：《微生物学通报》2021年第11期4232-4239,共8页Microbiology China

基　　金：国家自然科学基金(31670650);国家重点研发计划(2017YFD0600103-4-2);杨凌科技助力脱贫攻坚项目(2020-KJFP)。

摘　　要：【背景】在陕西省的毛泡桐腐木桩上观察和采集到一株绒毛状真菌子实体,编号为HMNWAFU-CF-HS002。【目的】为了确定该菌的分类地位,对其进行形态观察及分子鉴定。【方法】获取宏观彩色图像并对显微结构进行测量、统计和绘图。此外,用PDA培养基分离纯化该菌的培养物,并结合最大似然法、最大简约法和贝叶斯法进行rDNA ITS分子系统学研究。【结果】HMNWAFU-CF-HS002的形态特征与Punctularia atropurpurascens高度相似。系统发育分析将HMNWAFU-CF-HS002聚在P. atropurpurascens的单系分支中。【结论】结合形态学特征与系统发育结果,HMNWAFU-CF-HS002被鉴定紫黑点壳菌(P. atropurpurascens),为中国的新记录种。此外,毛泡桐为其新记录寄主。至此,Punctularia属下的3个种,均在中国有分布记录。[Background] A fluffy fungal fruitbody(No. HMNWAFU-CF-HS002) was collected from the rotten stumps of Paulownia tomentosa in Shaanxi province, China. [Objective] Morphological description and molecular identification were carried out to determine its taxonomic status. [Methods] Macroscopic features were imaged, while microstructures were measured, counted, and drawn. Living culture was isolated and purified with PDA, and phylogenetic analysis of r DNA internal transcribed spacer(ITS) was performed with maximum likelihood(ML), maximum parsimony(MP), and bayesian inference(BI) methods. [Results] The morphological characteristics of HMNWAFU-CF-HS002 were highly similar to those of Punctularia atropurpurascens. Phylogenetic analysis showed that HMNWAFU-CF-HS002 nested into the P. atropurpurascens clade. [Conclusion] Based on the morphological evidences and phylogenetic results, HMNWAFU-CF-HS002 was identified as P. atropurpurascens, a new record species in China. P. tomentosa is a new record host species for P. atropurpurascens. So far, all the three species of Punctularia have been recorded in China.

关 键 词：紫黑点壳菌 生物资源 形态学 rDNA ITS分子系统学 新记录种 

分 类 号：Q939.1[生物学—微生物学]