细菌性阴道病患者的加德纳菌临床分离株对苦参凝胶和甲硝唑的敏感性分析  

Sensitivity analysis of Gardnerella vaginalis clinical isolates of bacterial vaginosis to Kushen gel and metronidazole

作　　者：范琳媛 白会会 宗晓楠 张展 李婷 刘朝晖 FAN Linyuan;BAI Huihui;ZONG Xiaonan;ZHANG Zhan;LI Ting;LIU Zhaohui(Departmen of Gynecology,Beijing Obstetrics and Gynecology Hospital,Capital Medical University(Beijing Maternal and Child Health Care Hospital),Beijing 100026,China;Department of Clinical Laboratory,Beijing Obstetrics and Gynecology Hospital,Capital Medical University(Beijing Maternal and Child Health Care Hospital),Beijing 100026,China)

机构地区：[1]首都医科大学附属北京妇产医院(北京妇幼保健院)妇科,北京100026 [2]首都医科大学附属北京妇产医院(北京妇幼保健院)检验科,北京100026

出　　处：《中国性科学》2021年第11期51-54,共4页Chinese Journal of Human Sexuality

摘　　要：目的观察细菌性阴道病(BV)患者的加德纳菌(GV)临床分离株对苦参凝胶和甲硝唑的敏感性,为临床治疗BV提供科学依据。方法选取2018年12月至2019年12月首都医科大学附属北京妇产医院诊治的100例BV患者作为研究对象。采集其阴道分泌物,经分离纯化及16s rRNA测序鉴定得到GV临床菌株。通过微量肉汤稀释法检测GV临床菌株对甲硝唑及苦参凝胶的主要有效成分苦参总碱的敏感性。结果共鉴定出66株BV临床菌株,其中GV最多,占45.5%(30/66)。药敏实验结果显示这些GV菌株对甲硝唑的耐药率为63.33%(19/30)。苦参总碱对包括甲硝唑耐药菌株在内的所有GV临床菌株均具有抑制和杀伤作用,其最低抑菌浓度(MIC)和最低杀菌浓度(MBC)范围分别为0.16~2.50mg/mL和0.31~5.00mg/mL。结论苦参凝胶能够有效抑制和杀灭GV临床菌株,对于甲硝唑耐药的GV也具有良好的抗菌活性,说明苦参凝胶在治疗BV患者尤其是耐药患者方面具有较好的临床价值。Objective To observe the sensitivity of Gardnerella vaginalis clinical isolates of bacterial vaginosis(BV) to Kushen gel and metronidazole, thus providing scientific basis for clinical treatment of BV. Methods 100 patients with BV in Beijing Obstetrics and Gynecology Hospital, Capital Medical University from December 2018 to December 2019 were enrolled. The clinical strains were isolated from vaginal secretions of those patients, and then identified according to the 16 s rRNA gene. The sensitivity of Gardnerella vaginalis(GV) clinical strains to metronidazole and sophora flavescens alkaloids which is the main active ingredient of Kushen gel were determined by micro-broth dilution method. Results 66 clinical strains of BV were identified, with GV accounting for 45.5%(30/66). Drug sensitivity test showed that the resistance rate of these GV strains to metronidazole was 63.33%(19/30). The sophora flavescens alkaloids had inhibitory and killing effects on all clinical strains of GV including metronidazole resistant strains, and its minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) ranged from 0.16 to 2.50 mg/mL and 0.31 to 5.00 mg/mL,respectively. Conclusions Kushen gel can effectively inhibit and kill GV clinical strains, and maintain good antibacterial activity against metronidazole-resistant GV,indicating that Kushen gel has clinical promotion value in the treatment of BV patients, especially resistant patients.

关 键 词：细菌性阴道病 阴道加德纳菌 临床菌株 苦参总碱 苦参凝胶 甲硝唑 

分 类 号：R711[医药卫生—妇产科学]