香丹注射液和清开灵注射液联合用药对急性炎症血栓模型大鼠脏器的保护作用  

Synergistic Effect of Xiangdan Injection and Qingkailing Injection on Organ Injury Caused by Acute Inflammation and Thrombosis in Rats

作　　者：李春英[1] 易艳[1] 赵雍[1] 张宇实[1] 韩佳寅[1] 刘素彦[1] 梁爱华[1] LI Chun-ying;YI Yan;ZHAO Yong;ZHANG Yu-shi;HAN Jia-yin;LIU Su-yan;LIANG Ai-hua(Key Laboratory of Beijing for Identification and Safety of Chinese Medicine,State Key Laboratory of Innovative Natural Medicine and Traditional Chinese Medicine Injections,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所中药鉴定与安全性检测评估北京市重点实验室,创新天然药物与中药注射剂国家重点实验室,北京100700

出　　处：《中国实验方剂学杂志》2021年第23期80-87,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院基金项目(C12021A04801,ZZ-13-035-10,GH201919);中国中医科学院中药研究所基金项目(ZXKT20022,ZXKT19014);中国中医科学院中医药“一带一路”合作专项(GH201919)。

摘　　要：目的:基于血栓变化、炎性指标、脏器功能和病理改变等考察香丹注射液(Xiangdan injection,XDI)和清开灵注射液(Qingkailing injection,QKLI)联合用药在炎症血栓动物模型的治疗上是否具有协同增效作用。方法:100只雄性SD大鼠,根据体质量随机分为正常组,炎症血栓模型组,XDI组(2.5,5 mL·kg^(-1)),QKLI组(5,10 mL·kg^(-1)),XDI+QKLI组[(2.5+5)mL·kg^(-1),(2.5+10)mL·kg^(-1),(5+5)mL·kg^(-1),(5+10)mL·kg^(-1),每组10只大鼠。各组均腹腔注射给与相应注射液,每天1次,共4 d,正常组和模型组注射生理盐水。给药第2天,除正常组外,其余各组大鼠均造模,即先采用25 mg·kg^(-1)角叉菜胶腹腔注射入大鼠体内,16 h后大鼠再尾静脉注射50μg·kg^(-1)脂多糖(LPS)。注射LPS 24 h后,各组大鼠测定肝脏指数、肾脏指数,测定血小板(PLT)数量,测量血栓长度,全自动生化分析仪测定各项肝肾功能天门冬氨酸氨基转移酶(AST),丙氨酸氨基转移酶(ALT),碱性磷酸酶(ALP),肌酐(Crea)及尿素氮(BUN),酶联免疫吸附测定法(ELISA)检测炎性因子白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量,苏木素-伊红(HE)染色观察心、肝、肺、肾脏组织病理学变化,以及观察脏器病变分级情况。结果:与正常组比较,模型组大鼠PLT数量显著下降,大鼠尾部血栓长度显著增加,肝脏指数显著升高,肝肾功能ALT,ALP,BUN含量明显升高,IL-6和TNF-α含量明显升高(P<0.05,P<0.01),肝、肺、肾脏组织出现明显病理损伤(P<0.05,P<0.01)。与模型组比较,XDI 5 mL·kg^(-1)组明显降低大鼠血清ALT和ALP含量(P<0.05,P<0.01),QKLI 5,10 mL·kg^(-1)组大鼠血清ALT,ALP和TNF-α含量明显下降(P<0.05,P<0.01),XDI 5 mL·kg^(-1)和QKLI 10 mL·kg^(-1)单独用药均对LPS引起的肺脏病变损伤有明显减轻作用(P<0.05);XDI和QKLI合用后,明显降低大鼠的ALT,AST,ALP和TNF-α含量,尤其是XDI和QKLI合用(5+10)mL·kg^(-1)组效果最为明显(P<0.05,P<0.01),并且大鼠尾部血栓长度明Objective:To investigate the synergistic effect of Xiangdan injection(XDI)and Qingkailing injection(QKLI)in the treatment of inflammation and thrombosis animal model based on changes of thrombus,inflammatory indexes,organ function,and pathological changes.Method:A total of 100 male SD rats were randomly divided into a normal control group,a model group,XDI groups(2.5,5 mL·kg^(-1)),QKLI groups(5,10 mL·kg^(-1)),and XDI+QKLI groups[(2.5+5)mL·kg^(-1),(2.5+10)mL·kg^(-1),(5+5)mL·kg^(-1),and(5+10)mL·kg^(-1)]according to the body weight,with 10 rats in each group.Rats were treated correspondingly by intraperitoneal injection once a day for 4 days.The normal control group and the model group received normal saline.On the second day of administration,the model was induced in rats except those in the normal control group.Specifically,25 mg·kg^(-1)carrageenan was injected intraperitoneally into the rats,followed by an injection of 50μg·kg^(-1)lipopolysaccharide(LPS)through the tail vein 16 hours later.Twenty-four hours after LPS injection,the rats were detected for liver index,kidney index,the number of platelets(PLT),thrombus length,and biochemical indicators such as aspartate aminotransferase(AST),alanine aminotransferase(ALT),alkaline phosphatase(ALP),creatinine,and blood urea nitrogen(BUN).Enzymelinked immunosorbent assay(ELISA)was used to determine the content of inflammatory factors interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α).Hematoxylin-eosin(HE)staining was used to observe the histopathological changes of heart,liver,lung,and kidney,as well as the grading of organ injury.Result:Compared with the normal group,the model group showed decreased PLT,lengthened thrombus in the tail,increased liver index,elevated content of ALT,ALP,BUN,IL-6,and TNF-α(P<0.05,P<0.01),and damaged liver,lung,and kidney tissues(P<0.05,P<0.01).Compared with the conditions in the model group,XDI at 5 mL·kg^(-1)reduced serum ALT and ALP in rats(P<0.05,P<0.01),QKLI at 5 and 10 mL·kg^(-1)reduced serum levels ALT and ALP,and TN

关 键 词：香丹注射液 清开灵注射液 炎症 血栓 联合用药 

分 类 号：R2-0[医药卫生—中医学] R22