非酒精性脂肪性肝病动物模型研究进展  

Animal Models of Nonalcoholic Fatty Liver Disease:A Review

作　　者：汪丹丹 陈文静 贾步云 陈光亮[1] WANG Dan-dan;CHEN Wen-jing;JIA Bu-yun;CHEN Guang-liang(College of Integrative Medicine,First Affiliated Hospital,Anhui University of Chinese Medicine,Hefei 230012.China)

机构地区：[1]安徽中医药大学中西医结合学院,第一附属医院,合肥230012

出　　处：《中国实验方剂学杂志》2021年第23期245-250,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“重大新药创制”科技重大专项(2018ZX09739-010)。

摘　　要：非酒精性脂肪性肝病(NAFLD)是一种以肝细胞内脂质过度蓄积,与胰岛素抵抗和遗传易感密切相关的代谢应激性肝损伤。中医称其为"肝癖"。NAFLD影响着全球约25%的人,是世界卫生保健系统的主要负担,但其确切发病机制尚不清楚,开展NAFLD相关的基础研究具有重要的临床意义和社会价值。动物模型作为研究NAFLD的重要工具,对于明确NAFLD发生、发展的病理生理学机制尤为重要,近年来国内外关于NAFLD的造模方法不断更新,尤其是中医证候方面的模型取得了一定的发展。笔者通过查阅和整理近年来国内外的相关文献资料,对NAFLD动物模型复制方法进行了总结。该文对膳食诱导(高脂饲料、高脂高果糖饲料、高脂高胆固醇饲料、蛋氨酸胆碱缺乏饲料)模型、遗传基因缺陷[瘦素基因缺乏(Lep^(ob)/Lep^(ob))]小鼠、常染色体隐性糖尿病基因纯合子缺乏(ob/ob)小鼠、携带突变Alms1基因(foz/foz)小鼠,FATZO小鼠)+特殊饮食模型、中医证候(肝郁脾虚证、痰湿证、血瘀证、痰瘀互结证、气滞血瘀证)模型进行综述,并对其优缺点进行探讨,以期为制备更科学合理、经济便捷的NAFLD动物模型提供参考,为深入研究NAFLD的发病机制、预防与治疗NAFLD奠定基础。Nonalcoholic fatty liver disease(NAFLD)is a metabolic stress-induced liver injury characterized by excessive lipid accumulation in hepatocytes,which is closely related to insulin resistance and genetic susceptibility.It falls into the category of"liver lump"in traditional Chinese medicine(TCM).NAFLD affects about 25%of the population worldwide and has become a major burden of the world health care system However,its exact pathogenesis remains unclear.Conducting the basic research on NAFLD is of great clinical significance and social value.As an important tool for NAFLD research,animal model plays a particularly important role in clarifying the pathophysiological mechanism of NAFLD.In recent years,the modeling methods for NAFLD in China and abroad have been constantly updated,and in particular,certain progress has been made in the duplication of TCM syndrome models.By consulting and sorting out the relevant literature published in recent years in China and abroad,the author summarized the replication methods of NAFLD animal models This paper reviewed the advantages and disadvantages of models established via dietary induction(high-fa feed,high-fat and high-fructose feed,high-fat and high-cholesterol feed,and methionine choline-deficient feed),models with genetic defects[leptin-deficiency(Lep^(ob)/Lep^(ob)),autosomal recessive diabetes gene homozygous deficiency(ob/ob),Alms1 gene(foz/foz)mutation,and FATZO mice]and exposure to special diets,and models for TCM syndromes(liver depression and spleen deficiency syndrome,phlegm-dampness syndrome,blood stasis syndrome,combined phlegm and stasis syndrome,and qi stagnation and blood stasis syndrome),in order to provide reference for the preparation of more scientific,reasonable,economical,and convenient animal models of NAFLD,thus laying a foundation for in-depth study of the pathogenesis,prevention,and treatment of NAFLD.

关 键 词：非酒精性脂肪性肝病 动物模型 膳食诱导 遗传基因缺陷 中医证候 研究进展 

分 类 号：R2-0[医药卫生—中医学] R289