Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens  被引量:2

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作  者:Pengsong Li Yumingzi Wang Xin Yuan Xinying Liu Chunmao Liu Xiaofen Fu Dezhi Sun Yan Dang Dawn E.Holmes 

机构地区:[1]Beijing Key Laboratory for Source Control Technology of Water Pollution,Engineering Research Center for Water Pollution Source Control and Ecoremediation,College of Environmental Science and Engineering,Beijing Forestry University,Beijing 100083,China [2]Key Laboratory of Beijing Yanjing Beer Brewing Technology,Beijing Yan Jing Brewery Company Ltd.,Beijing 101300,China [3]MOST-USDA Joint Research Center for Biofuels,Beijing Engineering Research Center for Biofuels,Institute of New Energy Technology,Tsinghua University,Beijing 100084,China [4]Department of Physical and Biological Sciences,Western New England University,1215 Wilbraham Rd,Springfield,MA 01119,USA

出  处:《Environmental Science and Ecotechnology》2021年第2期3-10,共8页环境科学与生态技术(英文)

基  金:supported by the Fundamental Research Funds for the Central Universities[grant numbers BLX201934,2019ZY19];Beijing Municipal Education Commission through Innovative Transdisciplinary Program“Ecological Restoration Engineering”.

摘  要:In this study,an Escherichia coli(E.coli)whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein(GFP)reporter gene under the control of the ArsR1(GSU2952)regulatory circuit from Geobacter sulfurreducens.E.coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium.Under optimal test conditions,the Geobacter arsR1 promoter had a detection limit of 0.01 mM arsenite and the GFP expression was linear within a range of 0.03-0.1 mM(2.25-7.5 mg/l).These values were well below World Health Organization’s drinking water quality standard,which is 10 mg/l.The feasibility of using this whole-cell biosensor to detect arsenic in water samples,such as arsenic polluted tap water and landfill leachate was verified.The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry.This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples.

关 键 词:Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon 

分 类 号:TP2[自动化与计算机技术—检测技术与自动化装置]

 

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