动脉自旋标记及纵向弛豫时间定量评价大鼠肾脏冷缺血再灌注损伤的实验研究  

An experimental study of arterial spin labeling and T_(1)mapping evaluation of renal cold ischemia reperfusion injury in mouse models

作　　者：徐基磐 陈丽华[2] 任燕 袁仪忠 王振[3] 张全胜 胡占东[5] 朱锦霞 沈文[2] Xu Jipan;Chen Lihuan;Ren Yan;Yuan Yizhong;Wang Zhen;Zhang Quansheng;Hu Zhandong;Zhu Jinxia;Shen Wen(First Central Clinical College of Tianjin Medical University,Tianjin 300192,China;Department of Radiology,Tianjin First Central Hospital,Tianjin 300192,China;Organ Transplant Center,Tianjin First Central Hospital,Tianjin300192,China;Organ Transplantation Laboratory,Nankai University,Tianjin 300071,China;Department of Pathology,Tianjin First Central Hospital,Tianjin 300192,China;Department of Magnetic Resonance Research in Medical Field of Siemens(China)Co.Ltd,Beijing 100102,China)

机构地区：[1]天津医科大学一中心临床学院,300192 [2]天津市第一中心医院放射科,300192 [3]天津市第一中心医院器官移植中心,300192 [4]南开大学器官移植实验室,天津300071 [5]天津市第一中心医院病理科,300192 [6]西门子(中国)有限公司医疗业务领域磁共振科研部,北京100102

出　　处：《中华实验外科杂志》2021年第12期2363-2366,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(81873888)。

摘　　要：目的探讨动脉自旋标记(ASL)及纵向弛豫时间定量(T_(1)mapping)技术评价大鼠肾脏冷缺血再灌注损伤(CIRI)的应用价值。方法建立SD大鼠肾CIRI模型,根据冷缺血时间随机分为CIRI 0 h组、1 h组、2 h组、4 h组,每组20只。分别在术前、术后1 h、1、2、5 d选取5只行ASL及T_(1)mapping扫描,测量皮质肾血流量(RBF)值及肾皮、髓质的T_(1)值。取血进行生化检查,取大鼠左肾行病理学检查。采用重复测量方差分析比较各组磁共振成像(MRI)指标、生化指标的差异;非参数Kruskal-Wallis检验比较各组病理学评分的差异。Spearman和Pearson相关系数分别分析MRI指标与病理学评分的相关性。结果术后各组大鼠RBF值均较术前减低,最低点在术后1 h[(219.60±46.13)、(204.90±35.66)、(169.20±59.50)ml/min×100 g,F=13.600、29.283、24.491,P<0.05];除CIRI 4 h组外,各组RBF值术后5 d内恢复至术前水平(F=13.600、29.283、24.491,P值均>0.05)。CIRI 1 h、2 h、4 h组大鼠肾皮质T_(1)值在术后高于术前,5 d内分别升高至1616.00±70.73、1675.80±36.98、1663.20±55.44(F=3.123、14.660、11.662,P<0.05);CIRI 2 h、4 h组髓质T_(1)值5 d内分别升至1814.40±169.22、1868.10±174.41(F=11.340、10.500,P值均<0.01)。CIRI 1 h、2 h、4 h组在术后尿素氮(BUN)、丙二醛(MDA)、血肌酐(SCr)最高升高至20.95±1.79、18.95±2.08、109.24±3.29(F=99.903、55.180、51.370,P值均<0.01)。CIRI 2 h、4 h组病理评分明显高于术前(H=17.020、14.808,P均<0.01)。术后1 d大鼠RBF值与病理评分呈负相关(r=-0.670,P<0.01),术后2 d大鼠肾皮质T_(1)值与病理评分呈正相关(r=0.800,P<0.01);术后2 d大鼠肾髓质T_(1)值与病理评分呈正相关(r=0.840,P<0.01)。结论ASL和T_(1)mapping可以作为监测大鼠肾脏CIRI严重程度的无创检查方法。Objective To investigate the application value of arterial spin labeling(ASL)and T_(1)mapping assessment of the pathological changes in renal cold ischemia reperfusion injury(CIRI)mouse models noinvasively.Methods The kidney CIRI model of SD rats were established and divided into CIRI 0 h,1 h,2 h,4 h group according to cold ischemia durations randomly(20 rats in each group).Magnetic resonance imaging(MRI)was performed at the time points of before the surgery,1 hour after surgery,1,2 and 5 days after surgery using ASL and T_(1)mapping sequences.The renal cortical blood flow(RBF)and T_(1) values of the renal cortex and medulla were measured.Blood biochemical examination was performed.The left kidney of the resected rats was taken for pathological examination and the pathological score was calculated.The changes of RBF and T_(1) values were compared by repeated measurement analysis of variance.Nonparametric Kruskal-Wallis tests was used to compare the differences of histopathological scores.Spearman and Pearson correlation coefficient were used to analyze the correlation between RBF,T_(1) value,pathological scores.Results The RBF value in each group was lower than that before operation,and the lowest point at 1h after operation[(219.60±46.13),(204.90±35.66),(169.20±59.50)ml/min×100 g,F=13.600,29.283,24.491P<0.05,0.01,0.01],except for the CIRI 4 h group,RBF values in each group recovered to the preoperative level within 5 d after operation(F=13.600,29.283,24.491,all P values>0.05).The T_(1) value of renal cortex in CIRI 1 h,2h,4h group was higher than that before surgery,and increased to 1616.00±70.73,1675.80±36.98,1663.20±55.44 separately(F=3.123,14.660,11.662P<0.05,0.01,0.05).The T_(1) value of renal medulla in CIRI 2 h and 4 h groups increased to 1814.40±169.22 and 1868.10±174.41 within 5 d respectively(F=11.340,10.503P<0.01).The postoperative blood urea nitrogen(BUN),malondialdehyde(MDA),serum creatinine(SCr)in the group of CIRI 1 h,2 h,4 h maximum rise to 20.95±1.79,18.95±2.08,109.24±3.29(F=99.903,55

关 键 词：肾脏 冷缺血 缺血再灌注损伤 动脉自旋标记 纵向弛豫时间定量 

分 类 号：R692[医药卫生—泌尿科学]