依维莫司对大鼠移植肾动脉阻力指数的影响  

Effect of everolimus on renal allograft arterial resistance index in rats

作　　者：徐震[1] 桂泽平 郑明 刘绪忠[1] 顾民 谭若芸 Xu Zhen;Gui Zeping;Zheng Ming;Liu Xuzhong;Gu Min;Tan Ruoyun(Departmentof Urology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China;Departmentof Urology,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210011,China)

机构地区：[1]南京医科大学第一附属医院泌尿外科,210029 [2]南京医科大学第二附属医院泌尿外科,210011

出　　处：《中华实验外科杂志》2021年第12期2375-2377,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(82070769,81770751,81470981,81100532)。

摘　　要：目的观察依维莫司对大鼠移植肾动脉阻力指数的影响。方法将大鼠采取随机数字表法分为3组(每组12只):对照组(Fischer-Fischer)、手术组(Fischer-Lewis)及依维莫司处理组(Fischer-Lewis)。构建大鼠肾移植模型,于术后2、4、8周及12周分别采用多普勒超声法检测各组大鼠移植肾动脉阻力指数。收集术后12周大鼠血清及肾脏组织,检测各组大鼠血清肌酐及尿素氮值,过碘酸雪夫染色观察各组大鼠肾脏损伤情况。多组间比较采用单因素方差分析,两组间比较采用LSD检验。结果手术组大鼠血清肌酐[(105.18±8.61)μmol/L]及尿素氮[(29.47±2.99)mmol/L]明显高于对照组[(25.83±3.95)μmol/L,(7.72±0.67)mmol/L,P均<0.01],依维莫司组大鼠血清肌酐[(59.95±7.26)μmol/L]及尿素氮[(19.61±2.07)mmol/L]均低于手术组(P均<0.01);手术组大鼠可见明显肾脏损伤表现,依维莫司组大鼠肾脏损伤相对较轻。术后2周时手术组(0.70±0.05)及依维莫司组(0.65±0.04)大鼠肾动脉阻力指数明显高于对照组(0.52±0.05,P均<0.01);术后4~12周,手术组大鼠肾动脉阻力指数逐渐升高,而依维莫司组无明显变化;术后12周时,手术组肾动脉阻力指数(0.75±0.07)明显高于对照组(0.48±0.06,P<0.01);依维莫司组大鼠移植肾动脉阻力指数(0.58±0.05)明显低于手术组(P<0.01)。结论依维莫司对移植肾具有保护作用,同时可降低移植肾动脉阻力指数。Objective Vascular resistance index is an important indicator of renal allograft function.This study was to investigate the effect of everolimus on renal allograft arterial resistance index in rats.Methods Rats were divided into three groups(6 pairs in each group)using randomized number table method:control group(Fischer to Fischer),operation group(Fischer to Lewis)and everolimus treatment group(Fischer to Lewis).At 2,4,8 and 12 weeks after operation,the arterial resistance index of transplanted kidneys were measured by Doppler ultrasound.Serum andkidney tissues were collected 12 weeks after operation.Serum creatinine and urea nitrogen were detected.Periodic Acid-Schiff stain was used to evaluate the renal injury.Statistical comparisons between groups were determined using one-way ANOVA,followed by LSD test.Results The serum creatinine and urea nitrogen in the operation group[(105.18±8.61)μmol/L,(29.47±2.99)mmol/L]were significantly higher than those in the control group[(25.83±3.95)μmol/L,(7.72±0.67)mmol/L,bothP<0.01].The serum creatinine and urea nitrogen in the everolimus group[(59.95±7.26)μmol/L,(19.61±2.07)mmol/L]were lower than those in the operation group(bothP<0.01).The rats in the operation group showed obvious renal injury,while the everolimus group showed relatively mild renal injury.At 2 weeks after operation,the renal artery resistance index in operation group(0.70±0.05)and everolimus group(0.65±0.04)was significantly higher than that in control group(0.52±0.05,both P<0.01).At 4-12 weeks after operation,the renal artery resistance index of the operation group increased gradually,but there was no significant change in the everolimus group.At 12 weeks after operation,the renal artery resistance index of the operation group(0.75±0.07)was significantly higher than that of the control group(0.48±0.06)(P<0.01).Compared with the operation group,the renal artery resistance index of the everolimus group(0.58±0.05)was significantly decreased(P<0.01).Conclusion Everolimus can protect the transpla

关 键 词：肾移植 慢性排斥反应 血管阻力 依维莫司 

分 类 号：R699.2[医药卫生—泌尿科学]