补骨脂定对阿霉素心肌损伤的保护作用  

Protective effects of psoralidin against adriamycin induced myocardial injury

作　　者：梁振兴[1] 丁宝平 吴振 杨雯雯 陈颖[3] 陈科含 刘海[1] 徐敬[1] 杨阳[1,2] Liang Zhenxing;Ding Baoping;Wu Zhen;Yang Wenwen;Chen Ying;Chen Kehan;Liu Hai;Xu Jing;Yang Yang(Department of Cardiovascular Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Department of Life Sciences and Medicine,Northwestern University,Xi′an 710069,China;Department of Hematology,the First Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710061,China)

机构地区：[1]郑州大学第一附属医院心血管外科,450052 [2]西北大学生命科学与医学部,西安710069 [3]西安交通大学第一附属医院血液科,710061

出　　处：《中华实验外科杂志》2021年第12期2439-2443,共5页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(81700236)。

摘　　要：目的探究补骨脂定(PSO)是否对阿霉素(ADR)引起的心肌损伤具有保护作用。方法首先建立损伤合适的HL-1细胞模型,分为Control、ADR组(1、2、4、8μmol/L)、PSO+ADR组(10、20、50、100μmol/L),探究ADR最佳损伤浓度及PSO最佳保护浓度;通过检测细胞活力、活性氧(ROS)水平和培养液中乳酸脱氢酶(LDH)水平,探究PSO对ADR心肌损伤的保护作用;以6~8周雄性BALB/c小鼠为研究对象,构建ADR损伤模型,分为Sham、ADR、PSO+ADR组;使用小动物超声仪、全自动血生化分析仪、组织病理学染色检测并比较各组相关指标,阐明PSO对ADR心肌损伤的保护作用。两组间采用t检验比较差异显著性,两组以上组间采用单因素方差分析检验。结果细胞结果显示,与ADR组比较,20μmol/L PSO处理后的心肌细胞活力显著升高(t=6.694,P<0.05)、ROS水平降低(t=10.970,P<0.05)、培养液中LDH水平下降(t=4.172,P<0.05);动物实验结果表明,与ADR组比较,25 mg/kg PSO处理后可明显改善心功能,表现为小鼠心脏的每搏输出量(SV)和心输出量(CO)显著上升(t=4.547、2.850,P<0.05);血清中肌酸激酶(CK)水平下降(t=2.545,P<0.05);心肌纤维化程度明显减轻。结论PSO对ADR诱导的心肌损伤具有显著保护作用。Objective The goal of this study was to explore whether psoralidin(PSO)has a protective effect against myocardial injury induced by adriamycin(ADR).Methods An appropriate damage HL-1 cell model was established.The experiment was divided into Control group,ADR group(1,2,4,8μmol/L),and PSO+ADR group(10,20,50,100μmol/L)to explore the optimal concentration of ADR and the optimal protective concentration of PSO.The cell viability,reactive oxygen species(ROS)levels and lactate dehydrogenase(LDH)levels in the culture fluid was then detected;The 6-8 week old male BALB/c mice were used for in vivo study.Mice were divided into Sham group,ADR group,PSO+ADR group.Small animal ultrasound system,automatic blood biochemical analyzer,histopathological staining was used to detect and compare the relevant indicators to clarify the protective effects of PSO against ADR myocardial injury.T-test was used to compare the difference between the two groups,and one-way ANOVA was used to test more than two groups.Results The cell results showed that compared with the ADR group,20μmol/L PSO treatment could significantly increase the viability of myocardial cells(t=6.694,P<0.05),decrease the level of ROS(t=10.970,P<0.05)and LDH in the culture medium(t=4.172,P<0.05).Animal experiment results showed that compared with the ADR group,25 mg/kg PSO treatment can significantly improve cardiac function,manifested as increased stroke volume(SV)and cardiac output(CO)(t=4.547,2.850,P<0.05)and decreased serum creatine kinase(CK)levels(t=2.545,P<0.05).In addition,the reduced myocardial fibrosis was also observed in PSO-treated mice.Conclusion PSO conferred a significant cardioprotective effect against ADR injury.

关 键 词：补骨脂定 阿霉素 心肌损伤 

分 类 号：R285.5[医药卫生—中药学]