苹果坏死花叶病毒烟台分离物的鉴定与序列分析  

Identification and sequence analysis of Apple necrosis mosaic virus in Yantai isolates

作　　者：弟豆豆 宋来庆[1] 曹晓敏 胡慧 张硕[1] 张学勇[1] 姜中武[1,2] 赵玲玲[1,2] DI Doudou;SONG Laiqing;CAO Xiaomin;HU Hui;ZHANG Shuo;ZHANG Xueyong;JIANG Zhongwu;ZHAO Lingling(Yantai Academy of Agricultural Sciences,Shandong 265500;College of Life Science,Yantai University)

机构地区：[1]烟台市农业科学研究院,山东265500 [2]烟台大学生命科学学院

出　　处：《中国果树》2021年第9期42-47,51,I0002,共8页China Fruits

基　　金：国家苹果产业技术体系烟台综合试验站(nycytx-08);山东省外专双百计划项目(WST2018013);山东省重点研发项目(2018GHZ005);山东省重点研发项目(2018CXGC0207);山东省农业良种工程项目(2019LZGC007);山东省重点研发计划项目(2019GNC20601)。

摘　　要：采用逆转录聚合酶链反应(RT-PCR)技术,对在山东省烟台市采集的31份表现花叶症状的苹果幼叶进行了苹果坏死花叶病毒(Apple necrotic mosaic virus,ApNMV)和苹果花叶病毒(Apple mosaic virus,ApMV)检测。结果表明,ApNMV的检出率为87.1%,ApMV未检出,表明ApNMV与烟台地区苹果花叶病高度相关。从‘红将军’苹果和‘长富2号’苹果花叶样品中分别克隆出2条ApNMVRNA3基因组序列,GenBank登录号分别为MN911185和MN911186,这2条分离物核苷酸序列长度分别为1783 nt和1785 nt。序列比对结果显示,2条分离物核苷酸序列相似性为91.71%,与已报道的ApNMV RNA3核苷酸序列一致性为89.88%~96.64%。系统进化分析显示,不同来源的ApNMV亲缘关系较近,分离物之间没有表现出寄主特异性和地域分布规律。研究结果为该病的防治、流行规律的判断以及致病机理研究提供了理论依据。Using reverse transcription polymerase chain reaction(RT-PCR)technology,31 apple mosaic samples with mosaic symptoms collected in Yantai,Shandong Province were tested for Apple necrotic mosaic virus(Ap NMV)and Apple mosaic virus(Ap MV).The results showed that the detection rate of Ap NMV was 87.1%,and Ap MV was not detected,indicating that Ap NMV is highly related to apple mosaic disease in Yantai.Two Ap NMV RNA3 genomic sequences were cloned from’Red General’and’Changfu 2’apple leaves,the Gen Bank accession numbers were MN911185 and MN911186,respectively.The nucleotide sequences of these two isolates were 1783 nt and 1785 nt in length.The sequence comparison results showed that the nucleotide sequence similarity of the two isolates was 91.71%,and the nucleotide sequence identity with the reported Ap NMV RNA3 nucleotide sequence was 89.88%~96.64%.Phylogenetic analysis showed that Ap NMV from different sources had a close relationship,and the isolates did not exhibit host specificity and geographical distribution.The results of this study provide theoretical basis for the prevention and treatment of the disease,the judgment of epidemic laws and the study of the pathogenic mechanism.

关 键 词：苹果花叶病 苹果坏死花叶病毒 序列分析 

分 类 号：S436.661.19[农业科学—农业昆虫与害虫防治]