浙江省衢州地区鼠类携带莱姆病螺旋体研究  

Study of Borrelia burgdorferi in rodents in Quzhou area

作　　者：张建民[1] 占炳东 陈旭富 曹国平[1] 王双青[1] 吕磊[1] 杨瑞军[1] 叶承华[1] ZHANG Jian-min;ZHAN Bing-dong;CHENG Xu-fu;CAO Guo-ping;WANG Shuang-qing;LYU Lei;YANG Rui-jun;YE Cheng-hua(Quzhou Center for Disease Control and Prevention,Quzhou 324000,China)

机构地区：[1]浙江省衢州市疾病预防控制中心,衢州324000

出　　处：《中国人兽共患病学报》2021年第11期1053-1056,共4页Chinese Journal of Zoonoses

基　　金：浙江省医药卫生科技计划项目(No.2018KY881);衢州市科学技术局科技计划项目(No.20172091,No.20172092)。

摘　　要：目的了解衢州市鼠类携带莱姆病螺旋体状况以及莱姆病螺旋体基因型。方法采用针对莱姆病螺旋体5S~23S基因间隔区的巢式PCR方法检测鼠脾标本,对阳性样本进行基因测序及分析,构建系统发育树。结果在378份鼠标本中检出莱姆病螺旋体核酸阳性57份,阳性率15.08%。有Borrelia garinii(B.g)基因型、Borrelia valaisiana(B.v)基因型Borrelia spielmanii(B.sp)基因型。结论衢州境内鼠类存在至少3个型别莱姆病螺旋体感染,应加强该病原体的检测和莱姆病的防制。In order to understand the nature of infection and genotypes of Borrelia burgdorferi in rodents in Quzhou City,Zhejiang Province,nested PCR was used to amplify 5S-23S rRNA spacer fragments of B.burgdorferi from rodent spleen samples.The positive PCR products were sequenced and a phylogenetic tree was constructed.A total of 57 rodents were positive among 378 samples tested,with a prevalence of 15.08%.Sequence analysis of the amplification products showed identification of Borrelia garinii,Borrelia valaisiana and Borrelia spielmanii.The infection with at least three B.burgdorferi genotypes in rodents was confirmed in the Quzhou area.The findings of this study are necessary for the implementation of monitoring and prevention of Lyme disease.

关 键 词：伯氏疏螺旋体 鼠类 衢州地区 

分 类 号：R377[医药卫生—病原生物学]