外源单宁酸对茶叶中农药胁迫作用的缓解效应  

Mitigating effect of exogenous tannic acid on the stress of pesticides in tea

作　　者：刘尚可 王越 潘灿平[2] 贾厚振 刘铭钰 邹楠 LIU Shangke;WANG Yue;PAN Canping;JIA Houzhen;LIU Mingyu;ZOU Nan(Key Laboratory of Pesticide Toxicology&Application Technique,College of Plant Protection,Shandong Agricultural University,Taian 271018,Shandong Provine,China;Innovation Center for Pesticide Research,Department of Applied Chemistry,College of Science,China Agricultural University,Beijing 100193,China;Tea Research Institute of Shandong Academy of Agricultural Sciences,Ji'nan 250131,China;Agricultural Technology Extension Center of Linyi City,Shandong Province,Linyi 276007,Shandong Provine,China)

机构地区：[1]山东农业大学植物保护学院农药毒理与应用技术重点实验室,山东泰安271018 [2]中国农业大学理学院应用化学系农药创新研究中心,北京100193 [3]山东省农业科学院茶叶研究所,济南250131 [4]山东省临沂市农业技术推广中心,山东临沂276007

出　　处：《农药学学报》2021年第6期1150-1158,共9页Chinese Journal of Pesticide Science

基　　金：国家重点研发计划项目(2018YFC1604401);国家自然科学基金青年基金(31801781).

摘　　要：农药是控制病虫草害的重要手段,但农药也会对植物的氧化系统及营养品质产生影响。基于单宁酸的天然属性和结构特点,研究了在农药诱导的氧化胁迫下,叶面施用单宁酸对茶叶抗氧化系统和营养品质的潜在作用。与单独施用农药相比,加施10 mg/L单宁酸显著提高了茶叶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)及抗坏血酸过氧化物酶(APX)活性,其中POD活性分别是空白对照、吡唑醚菌酯单独施用的4.69倍和3.13倍,APX活性比单独施用吡虫啉、联苯菊酯和吡唑醚菌酯分别提高了340.74%、114.71%和271.70%。此外,单宁酸显著提高了茶叶总抗氧化能力,提高了脯氨酸、还原型谷胱甘肽和茶多酚含量,降低了膜脂过氧化产物丙二醛的含量。单宁酸还提高了茶叶中维生素C、可溶性蛋白、咖啡碱和游离氨基酸的含量。综上,外源单宁酸能够激活茶叶抗氧化酶活性,影响抗氧化非酶物质的积累,缓解茶叶中农药胁迫带来的氧化损伤,最终提高茶叶品质。Pesticides are important tools to manage diseases,insect pests and weeds.And they can also affect the oxidation system and nutritional quality of plants.Based on the natural properties and structural characteristics of tannin acid,the potential effects of foliar application of tannin acid on the antioxidant system and nutritional quality of tea under oxidative stress induced by pesticides were studied.Compared with the pesticide application alone,the addition of 10 mg/L tannic acid significantly increased tea superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),and ascorbate peroxidase(APX)activity,in which the POD activity is 4.69 times and 3.13 times that of the control and pyraclostrobin treatment respectively.Compared with the application of imidacloprid,bifenthrin and pyraclostrobin alone,the APX activity is increased by 340.74%,114.71%and 271.70%.In addition,tannin acid significantly increased the total antioxidant capacity of tea and the content of proline.Tannin acid reduced glutathione,tea polyphenols,the content of malondialdehyde and membrane lipid peroxidation products.Tannic acid also increases the content of vitamin C,soluble protein,caffeine and free amino acids in tea.In summary,exogenous tannin acid can activate antioxidant enzyme activity of tea leaf,affect the accumulation of antioxidant non-enzymatic substances,alleviate the oxidative damage caused by pesticide stress in tea,and improve the quality of tea ultimately.

关 键 词：单宁酸 茶叶 农药胁迫 氧化损伤 抗氧化酶活性 

分 类 号：S481.3[农业科学—农药学] S571.1[农业科学—植物保护]