基于关联分析的吴茱萸与8味甘味药配伍作用规律研究  

Study on the compatibility of Euodia rutaecarpa with 8 sweet-taste herbs based on association analysis

作　　者：李丽[1] 孙立丽[1] 任晓亮[1] 刘亚男[1] 窦志英[1] 刘虹 LI Li;SUN Lili;REN Xiaoliang;LIU Yanan;DOU Zhiying;LIU Hong(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Tianjin State Key Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学,天津301617 [2]天津中医药大学中医药研究院天津市现代中药重点实验室,天津301617

出　　处：《天津中医药大学学报》2021年第6期770-777,共8页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(81803955)。

摘　　要：[目的]以关联分析和高效液相色谱法(HPLC)探讨甘味中药在配伍过程中对吴茱萸的作用规律,以期为吴茱萸配伍机制研究提供参考。[方法]通过收集整理含有吴茱萸的处方,利用Apriori算法挖掘其潜在配伍规律并获取常与吴茱萸配伍的甘味中药;采用HPLC分析法,检测配伍前后吴茱萸中3种主要成分柠檬苦素、吴茱萸碱、吴茱萸次碱在提取物中的含量变化。[结果]8种甘味中药均可不同程度的降低吴茱萸中3种主要成分的含量;黄芪使3种有效成分的含量明显下降,甘草可明显地降低吴茱萸碱和吴茱萸次碱的含量但对柠檬苦素的作用较小。[结论]甘味中药能够降低吴茱萸中主要毒性成分兼活性成分的含量进而降低其毒性;黄芪对3种成分的降低作用最显著,甘草有良好的“减毒存效”作用。本研究应用数据挖掘技术结合HPLC发掘吴茱萸配伍规律并进行分析,为探索药物配伍的合理性和规律性提供参考。[Objective]In this study,the association rule analysis and high performance liquid chromatography(HPLC)were implemented to explore the effect of sweet-taste herbs on Euodia rutaecarpa in the process of compatibility,in order to provide a reference for the study of Euodia rutaecarpa compatibility mechanism.[Methods]The Apriori algorithm was used to explore the potential compatibility law and obtain the sweet-taste herbs that are often compatible with Euodia rutaecarpa.HPLC method was applied to detect the content variation of limonoids,evodiamine and rutaecarpine of Euodia rutaecarpa in compatibility.[Results]The 8 kinds of sweet-taste herbs can reduce the content of three main components in Euodia rutaecarpa in different degrees;Astragalus membranaceus significantly reduced the content of 3 active components,and licorice can obviously reduce the content of evodiamine and rutaecarpine but has no significant effect on limonoids.[Conclusion]Sweet-taste herbs can reduce the content of main toxic and active ingredients in Euodia rutaecarpa,and then reduce its toxicity.Astragalus membranaceus has the most significant effect of reducing the three components,glycyrrhiza has a good effect of“reducing toxicity and saving effect”.In this study,an approach exploiting the data mining techniques and HPLC to explore the rationality of compatibility for Euodia rutaecarpa is here proposed.This strategy has the potential to provides a reference for the study of the rationality and regularity of Chinese herbal medicines compatibility.

关 键 词：吴茱萸 关联分析 甘味药 配伍 

分 类 号：R285.1[医药卫生—中药学]