携带DEV-gB基因重组质粒减毒沙门氏菌在鸭免疫器官中的表达动态  

Expression Dynamics of Attenuated Salmonella Carrying Recombinant Plasmid DEV-gB in Duck Immune Organs

作　　者：刘妍罕 王相金 胡安东 曾茂芹 杨颖[1,3] 温贵兰 程振涛[1,3] 文明 LIU Yanhan;WANG Xiangjin;HU Andong;ZENG Maoqin;YANG Ying;WEN Guilan;CHENG Zhen-tao;WEN Ming(College of Animal Science,Guizhou University,Guiyang,Guizhou 550025,China;Department of Animal Husbandry and Aquaculture,Guizhou Vocational College of Agriculture,Qingzhen,Guizhou 551400,China;Guizhou Research Center of Engineering Technology for Animal Biological Products,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州农业职业学院畜牧水产系,贵州清镇551400 [3]贵州省动物生物制品工程技术研究中心,贵州贵阳550025

出　　处：《山地农业生物学报》2021年第6期37-43,共7页Journal of Mountain Agriculture and Biology

基　　金：国家自然科学基金项目(31560703);贵州省百层次创新型人才项目(黔科合人才[2016]4009);贵州省科技平台及人才团队计划项目(黔科合平台人才[2018]5253)。

摘　　要：为明确含DEV-gB基因重组减毒沙门氏菌在鸭免疫器官中的分布动态,本研究将重组减毒沙门氏菌口服接种鸭(即重组细菌组),同时设立重组质粒组、弱毒疫苗组和空白对照组,于免疫后第7、14、21、28、35、42和49天时,捕杀试验鸭,采集鸭脾脏、胸腺、法氏囊和哈德氏腺等免疫器官,采用间接免疫荧光法进行DEV-gB抗原检测观察,结果显示:空白对照组鸭免疫器官在7个时间点均未检测到DEV-gB抗原信号;免疫后,重组细菌组、弱毒疫苗组和重组质粒组鸭免疫器官均能检测到DEV抗原阳性信号,但含DEV抗原阳性信号细胞数量从多到少的组别顺序是弱毒疫苗组、重组细菌组和重组质粒组,且重组细菌组与弱毒疫苗组差异不显著(P>0.05);检出含DEV抗原阳性信号的免疫器官先后顺序是脾脏、法氏囊、哈德氏腺和胸腺,而含DEV抗原阳性信号细胞数量从多到少的免疫器官顺序是脾脏、法氏囊、胸腺和哈德氏腺。由此可见,本研究构建的携带DEV-gB基因重组减毒沙门氏菌能在鸭免疫器官中得到良好的表达,为该重组细菌疫苗的临床应用奠定了理论基础。To determine the distribution dynamics of recombinant attenuated salmonella carring DEV-gB gen in the immune organs of ducks,and inoculated with recombinant bacteria,recombinant plamid,attenuated vaccine and sterile PBS silution(blank control),respectively.The experimental ducks were killed at the 7^(th),14^(th),21^(th),28^(th),35^(th),42^(th) and 49^(th) day after immunization,and the spleen,thymus,Fabricius bursa and Hardy's gland were collected,and detected for DEV-gB antigen by indirect immunofluorescence method.The results showed that no signal of DEV-gB antigen was detected in duck immune organs of blank control group at every time-point,but positive signal presented in the other three groups.And the cell number with DEV-gB positive signal in the recombinant bacteria group was,the most,followed by attenuated vaccine group and recombinant plasmid group,but there was no significant difference between the recombinant bacteria group and the attenuated vaccine group.The order of the immune organs detected with positive signal of DEV-gB antigen were respectively spleen,Fabricius bursa,Hardy's gland and thymus,while the cell number with DEV-gB positive signal in spleen were the most,followed by Fabricius bursa,thymus and Hardy's gland.Therefore,the recombinant attenuated Salmonella carrying DEV-gB gene could be well expressed in duck immune organs,laying a theoretical basis for the clinical application of the recombinant bacterial vaccine.

关 键 词：DEV 重组减毒沙门氏菌 鸭 表达动态 

分 类 号：S8[农业科学—畜牧兽医]