苷芝宝蜜茶饮对小鼠急性酒精性肝损伤的保护作用  

Protective Effect of Ganzippo on Acute Alcoholic Liver Injury in Mice

作　　者：叶敏贞[1] 卞仪泽 郑宁宁 欧伟杰 李雪莹 张南文[2] YE Min-zhen;BIAN Yi-ze;ZHENG Ning-ning;OU Wei-jie;LI Xue-ying;ZHANG Nan-wen(Sanming Integrated Traditional Medicine Hospital,Sanming 365001,China;School of Pharmacy,Fujian Medical University,Fuzhou 350122,China;The First Affiliated Hospital of Fujian Medical University,Liver Research Center,Fuzhou 350005,China;School of Traditional Chinese Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]三明市中西医结合医院,福建三明365001 [2]福建医科大学药学院,福建福州350122 [3]福建医科大学附属第一医院肝病中心,福建福州350005 [4]福建中医药大学中医学院,福建福州350122

出　　处：《海峡药学》2021年第11期18-21,共4页Strait Pharmaceutical Journal

基　　金：三明市科技计划项目(编号2018-S-3);福建省科技特派员项目(2019B042);国家级大学生创新创业训练计划项目(编号:202010393014);福建中医药大学校管课题(编号XB2019099)。

摘　　要：目的通过动物实验观察苷芝宝蜜茶饮预处理对酒精性肝损伤的保护作用,为苷芝宝蜜茶饮保肝功能提供科学依据。方法25只ICR雄性小鼠随机分为5组:对照组、模型组和苷芝宝低、中、高剂量组,每组5只,分别给予超纯水及不同浓度的苷芝宝溶液灌胃,1日1次,持续7天。末次灌胃结束后8 h,对照组小鼠给予超纯水灌胃,其余各组小鼠给予红星二锅头灌胃,诱导急性酒精性肝损伤。所有小鼠进食不禁水16 h取血清用于转氨酶测定,取肝组织标本以HE和油红O染色法观察形态学改变。结果与模型组比较,苷芝宝组小鼠肝脏指数、血清转氨酶水平下调,具有显著统计学差异(P<0.001),病理学实验可见肝组织中的炎症细胞和脂质沉积情况均较模型组下降。结论苷芝宝蜜茶饮预处理具有保护小鼠急性酒精性肝损伤的作用。OBJECTIVE To observe the protective effect of Ganzippo pretreatment on alcoholic liver injury through animal experiments,and to provide scientific basis for the liver protection function of Ganzippo honey tea.METHODS 25 ICR male mice were randomly divided into 5 groups:control group,model group and Ganzippo low,medium and high dose groups,5 mice in each group.They were irrigated with ultrapure water and different concentrations of Ganzippo solution(qd for 7 days,ig).8 hours after the last gavage,mice in the control group was gavaged with ultrapure water,and mice in the remaining groups were gavaged with Red Star Erguotou to induce acute alcoholic liver injury.All mice were fed with water for 16 hours to obtain serum for transaminase determination,and liver tissue specimens were taken to observe the morphological changes by HE and Oil Red O staining.RESULTS Compared with the model group,the liver index and serum transaminase levels of the mice in the Ganzippo group were down-regulated,with significant statistical differences(P<0.001).Pathological experiments showed that the inflammatory cells and lipid deposition in liver tissues were all decreased in Ganzippo group.CONCLUSION Ganzippo pretreatment can protect mice from acute alcoholic liver injury.

关 键 词：小鼠 酒精 肝脏 损伤 急性 

分 类 号：R965[医药卫生—药理学]