油茶高产与低产无性系代谢表达谱标记鉴别  

Identification of Potential Metabolic Markers for the High and Low Yield Clones of Camellia oleifera

作　　者：曹汉洋 何祯祥[2] 李志真[3] 卢世明 郑登耿 陈耀升 赖标祯 CAO Hanyang;HE Zhenxiang;LI Zhizhen;LU Shiming;ZHENG Denggeng;CHEN Yaosheng;LAI Biaozhen(Shaxian Shuinan state-owned forest farm,Shaxian 365500,Fujian,China;School of life sciences,Nanjing University,Nanjing 210046,Jiangsu,China;Fujian Academy of Forestry Sciences,Fuzhou 350012,Fujian,China;Fuzhou botanical garden,Fuzhou 350012,Fujian,China)

机构地区：[1]福建省沙县水南国有林场,福建沙县365500 [2]南京大学生命科学学院,江苏南京210046 [3]福建省林业科学研究院,福建福州350012 [4]福州植物园,福建福州350012

出　　处：《福建林业科技》2021年第4期8-12,共5页Journal of Fujian Forestry Science and Technology

基　　金：福建省三明市林业科技项目(明林综(2019)65号)。

摘　　要：于2020年,在福建省沙县水南国有林场的油茶无性系评比试验林中选择3株高产油茶树和3株低产油茶树,采用GC-MS技术开展与油茶产量性状相关的代谢表达谱分析与代谢标记鉴定。研究结果表明:从高产组和低产组代谢表达谱中筛选出2种含量达显著性差异的化合物:角鲨烯的相对含量高产组为4.07%,低产组为35.87%,低产组显著高于高产组(P<0.01);保留时间为17.227 min的化合物A在高产组中相对含量为31.85%,而在低产组中没有检测到。这些代谢表达物可能作为油茶优良无性系选择的代谢标记,为油茶高产品种的选择提供新方法。In 2020,three high yield individuals and three low yield individuals were selected from the clone experimental forest of Camellia oleifera L.in Shuinan State-owned Forest Farm,Shaxian,Fujian Province.The metabolic expression profile analysis and metabolic marker identification related to the yield traits of oil tea were carried out by GC-MS.Results showed that two compounds with significant difference were screened from the metabolic expression profiles between high and low yield group.The relative content of squalene in high yield group was 4.07%,and that in low yield group was 35.87%,which was significantly higher than that in high yield group(P<0.001);the relative content of compound a with retention time of 17.227 min in high yield group was 31.85%,which was not detected in low yield group.These metabolites could be used as metabolic markers for selection of high yield clones of C.oleifera,which may provide a new method for the selection of high yield varieties of C.oleifera.

关 键 词：油茶 GC-MS分析 代谢产物标记 

分 类 号：S794.4[农业科学—林木遗传育种]