4种Keggin型磷钼酸盐对蘑菇酪氨酸酶活性和黑色素生成的抑制及抗氧化作用  

Inhibitory Effect of Four Kinds of Keegin-type Phosphomolybdate on Tyrosinase and Melanin Formation and Its Antioxidant Activities

作　　者：帅蝶 赵美娟 陈丙年 王力[1] SHUAI Die;ZHAO Meijuan;CHEN Bingnian;WANG Li(College of Food and Biological Engineering,Jimei University,Xiamen 361021,China;Xiamen University Hospital,Xiamen 361005,China)

机构地区：[1]集美大学海洋食品与生物工程学院,厦门361021 [2]厦门大学医院,厦门361005

出　　处：《高等学校化学学报》2021年第12期3579-3588,共10页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21871110);福建省自然科学基金(批准号:2020J01674)资助.

摘　　要：合成并表征了4种过渡金属钒取代的Keggin型磷钼酸盐Na^(^(3+))nPMo_(12−n)V_(n)O_(40)(PMo_(12−n)V_(n))(n=2,3,4,5).酶动力学实验结果表明,4种多酸对酪氨酸酶的抑制类型为可逆的混合型抑制,过渡金属钒取代的个数会影响酪氨酸酶的抑制效果.当所加酶量为500 U/mL时,PMo_(10)V_(2),PMo_(9)V_(3),PMo_(8)V_(4)和PMo_(7)V_(5)对酪氨酸酶的半抑制率(IC50)分别为(7.046±0.506),(12.128±0.574),(12.362±0.802)和(9.860±1.490)mmol/L.分子对接研究表明,化合物与酪氨酸酶之间主要存在氢键和范德华力.细胞实验结果表明,在0~200μmol/L范围内,4种多酸未对B16黑素瘤细胞产生细胞毒性,且能显著抑制黑色素的生成.此外,1,1-二苯基-2-三硝基苯肼(DPPH)与2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除实验显示,4种多酸具有良好的抗氧化能力,清除DPPH自由基能力优于ABTS.Four kinds of transition metal V substituted Keggin type phosphomolybdate Na^(3+)nPMo_(12−n)V_(n)O_(40)(abbreviated as PMo_(12−n)V_(n))(n=2,3,4,5)were synthesized and characterized.The kinetic enzymatic method suggested that the inhibition type of four compounds on tyrosinase was reversible mixed inhibition,and the number of transition metal vanadium substitution would affect the inhibition effect of tyrosinase.When the enzyme content was 500 U/mL,the inhibition rates of PMo_(10)V2,PMo_(9)V_(3),PMo_(8)V_(4) and PMo_(7)V_(5) on tyrosinase were(7.046±0.506),(12.128±0.574),(12.362±0.802),(9.860±1.490)mmol/L,respectively.The molecu⁃lar docking experiment confirmed that there was hydrogen bond and van der Waals force interaction between the compound and tyrosinase.The four compounds did not produce cytotoxic effects on B16 melanoma cells,and could significantly inhibit the production of melanin.In addition,1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl(DPPH)and 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulpho⁃nate)(ABTS)free radical scavenging experiments confirmed that polyoxometalates had good antioxidant capacity.What’s more,they have stronger DPPH radical scavenging ability.

关 键 词：多金属氧酸盐 酪氨酸酶 酶动力学 分子对接 黑色素 抗氧化 

分 类 号：O614.5[理学—无机化学] Q55[理学—化学]