表面定向磁性印迹聚合物的制备及对槲皮素的选择性识别  

Preparation of Surface Oriented Magnetically Imprinted Polymers and the Selective Recognition of Quercetin

作　　者：李菲[1] 李小轩 李一峻[2] 何锡文[2] 陈朗星[2] 张玉奎[2,3] LI Fei;LI Xiaoxuan;LI Yijun;HE Xiwen;CHEN Langxing;ZHANG Yukui(College of Chemical Engineering and Materials Science,Shandong Normal University,Jinan 250000,China;College of Chemistry,Tianjin Key Laboratory of Biosensing and Molecular Recognition,Nankai University,Tianjin 300071,China;Dalian Institute of Chemical Physics,Chinese Academy of Science,Dalian 116023,China)

机构地区：[1]山东师范大学化学化工与材料科学学院,济南250000 [2]南开大学化学学院,生物传感与分子识别天津市重点实验室,天津300071 [3]中国科学院大连化学物理研究所,大连116023

出　　处：《高等学校化学学报》2021年第12期3606-3614,共9页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:22074067)资助.

摘　　要：利用硼酸功能化的磁性碳纳米管作为反应基质,采用一种简便、绿色的硼酸亲和表面定向印迹法制备了槲皮素磁性分子印迹聚合物,并将其应用于银杏叶提取物中槲皮素的特异性识别.透射电子显微镜、X射线光电子能谱仪、X射线衍射及振动样品磁强计测试结果表明,制备的分子印迹聚合物具有良好的形貌和晶型结构.吸附实验结果表明,该分子印迹聚合物对模板分子槲皮素具有较好的吸附容量(4.57μg/mg)、良好的印迹效果(IF=8.44)和再生能力.对实际中药样品银杏叶提取物的吸附实验结果表明,所建立的方法能达到预期的槲皮素检测效果,可作为中药有效成分槲皮素的特异性识别工具.Flavonoids are one of the most important compounds,which have been successfully used in medi⁃cine in ancient times because of their unique antioxidant and anti-inflammatory effects.Quercetin is a polyphenol compound,which contains cis-diol structure and belongs to flavonol.It is found in a variety of foods,including vegetables and fruits,such as tea,cocoa powder,cranberries,kale,celery,cauliflower,let⁃tuce,tomatoes and carrots.Quercetin has the ability of scavenging free radicals and binding transition metal ions so that it can inhibit and reduce oxidative stress and related damage.Quercetin also has a variety of biolog⁃ical activities,such as antioxidant,antiviral,anti-tumor and so on.In addition,quercetin shows the potential to treat and prevent a variety of diseases,such as cancer,osteoporosis,asthma,Alzheimer's disease,diabe⁃tes and skin diseases.Molecularly imprinted polymers(MIPs)are chemically synthesized receptors with pre-designed binding sites and rigidity to target molecules and have important applications in many fields,such as chemical sensing,separation,catalysis and disease diagnosis.Boric acid is an important functional monomer which can reversibly bind nucleosides,sugars,polysaccharides and glycoproteins to cis-diol compounds in co⁃valent imprinting.In recent years,boric acid functional materials have attracted more and more attention.Con⁃sidering the importance of selectivity and sensitivity to the efficiency of detection methods,a new imprinting strategy,called boric acid affinity surface oriented imprinting,was proposed by combining the covalent bin-ding of boric acid affinity with molecular imprinting technique.It is used to prepare high performance molecu⁃larly imprinted materials.This directed imprinting strategy is based on the interaction between the functional solid matrix and the template.In the process of template immobilization,the covalent interaction(boric acid af⁃finity)is more stable than the non-covalent interaction,and the imprinting process can produce a more un

关 键 词：表面定向印迹 磁性碳纳米管 槲皮素 识别 

分 类 号：O657[理学—分析化学]