扶正消瘤颗粒对乳腺癌SKBR-3细胞HER-2 mRNA及蛋白表达的影响  

Effect of Fuzheng Xiaoliu Granules on HER-2 mRNA and Protein Expressions in SKBR-3Cells of Breast Cancer

作　　者：岳双冰[1] 田欢[1] 甘洁文[1] 林洪[1] 李琴[1] 金宇[1] 张子理[1] YUE Shuangbing;TIAN Huan;GAN Jiewen;LIN Hong;LI Qin;JIN Yu;ZHANG Zili

机构地区：[1]深圳市第二人民医院,广东深圳518000

出　　处：《新中医》2021年第22期130-134,共5页New Chinese Medicine

基　　金：深圳市科技计划项目(JCYJ20160425103035168)。

摘　　要：目的:观察扶正消瘤颗粒对乳腺癌SKBR-3细胞HER-2 mRNA及蛋白表达的影响。方法:将30只SD大鼠随机分成扶正消瘤颗粒L组、M组、H组,3组大鼠分别予1倍、2倍、3倍量的扶正消瘤颗粒灌胃3 d后,制备扶正消瘤颗粒的大鼠含药血清。体外培养HER-2阳性的人乳腺癌SKBR-3细胞,对数生长期细胞随机取5组,分别是空白对照组、赫赛汀(Herceptin)组及扶正消瘤颗粒的大鼠含药血清低、中、高剂量组(L组、M组、H组),进行相应干预。采用实时PCR检测HER-2 mRNA表达,Western Blot检测HER-2蛋白表达。结果:扶正消瘤颗粒3个剂量组及Herceptin组的HER-2 mRNA含量与空白对照组比较,在24 h、48 h、72 h 3个时间点均有下降(P<0.01),提示3种剂量的大鼠含药血清和Herceptin均能抑制SKBR-3细胞的HER-2 mRNA表达。L组24 h和48 h的HER-2 m RNA含量及M组48 h的HER-2 mRNA含量与Herceptin组比较,差异均无统计学意义(P>0.05);L组72 h,M组24 h及72 h,H组24 h、48 h、72 h的HER-2 m RNA表达量与Herceptin组比较,差异均有统计学意义(P<0.01,P<0.05)。扶正消瘤颗粒3个剂量组及Herceptin组24 h的HER-2 mRNA含量均最低,48 h的HER-2 mRNA含量较24 h升高,72 h的mRNA含量又降低。与空白对照组进行比较,Herceptin组和L组的SKBR-3细胞HER-2蛋白量均下降,差异均有统计学意义(P<0.01);与Herceptin组比较,M组及H组的HER-2蛋白量较高,差异均有统计学意义(P<0.01)。L组的HER-2蛋白量与Herceptin组比较,差异无统计学意义(P>0.05)。结论:扶正消瘤颗粒的大鼠含药血清能明显抑制乳腺癌SKBR-3细胞HER-2 m RNA表达和HER-2蛋白表达,其抑制作用较Herceptin要差一些,扶正消瘤颗粒低剂量的含药血清效果更好。Objectives:To observe the effect of Fuzheng Xiaoliu granules on human epidermal growth factor receptor 2(HER-2) mRNA and protein expressions in SKBR-3 cells of breast cancer. Methods:A total of 30 SD rats were randomly divided into the Fuzheng Xiaoliu granules low-dose group(Group L), median-dose group(Group M) and high-dose group(Group H). In the three groups,rats with medicated serum containing Fuzheng Xiaoliu granules were prepared by Fuzheng Xiaoliu granules by gavage at the doses of one, two and three times respectively. HER-2-positive human breast cancer SKBR-3 cells were cultured in vitro. Rats with cells in the logarithmic growth phase were randomly divided into five groups,with the blank control group,the Herceptin group and the three groups of rats with medicated serum containing Fuzheng Xiaoliu granules at three different doses,and were given corresponding intervention. The HER-2 m RNA expressions were detected by real-time polymerase chain reaction(PCR),and the HER-2 protein expressions by Western Blot. Results:The HER-2 mRNA contents in the three groups of Fuzheng Xiaoliu granules and the Herceptin group were decreased at 24 h,48 h and 72 h when compared with those in the blank control group(P<0.01),suggesting that rats with medicated serum at three doses and Herceptin could inhibit the HER-2 mRNA expressions in SKBR-3 cells. There was no significant difference being found in the comparison of HER-2 m RNA contents at 24 h and 48 h between Group L and the Herceptin group,and of HER-2 mRNA contents at 48 h between Group M and the Herceptin group(P>0.05). The comparisons between HER-2 mRNA expressions in Group L at 72 h, Group M at 24 h and 72 h,Group H at 24 h,48 h and 72 h and those in the Herceptin group showed significant differences(P<0.01,P<0.05). The HER-2 mRNA contents at 24 h in the three groups of Fuzheng Xiaoliu granules at three doses and the Herceptin group were the lowest,the HER-2 mRNA contents at 48 h were increased when compared with those at 24 h,and decreased at 72 h. The HER-2 protei

关 键 词：乳腺癌 扶正消瘤颗粒 赫赛汀 SKBR-3细胞株 HER-2 mRNA HER-2蛋白 

分 类 号：R285.5[医药卫生—中药学]