苯菌灵处理条件下内生真菌感染对羊草生长的影响  

Effect of endophytic fungi infection on the growth of Leymus chinensis under benomyl treatment

作　　者：刘慧 崔光杰 李明明 朱元刚 张秀玲 LIU Hui;CUI Guang-jie;LI Ming-ming;ZHU Yuan-gang;ZHANG Xiu-ling(College of Life Sciences,Dezhou University,Dezhou 253023,Shandong,China)

机构地区：[1]德州学院生命科学学院,山东德州253023

出　　处：《生态学杂志》2021年第11期3570-3576,共7页Chinese Journal of Ecology

基　　金：德州学院科学研究基金项目(2019xjrc317);国家自然科学基金项目(32001103)资助。

摘　　要：Epichlo3内生真菌和丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)同时感染宿主禾草的现象在自然和农业生态系统中广泛存在,目前的研究主要集中于内生真菌和某一单一人工接种的AMF对宿主禾草生长的影响,少有研究关注内生真菌和自然生境中AMF互作对宿主的影响。在盆栽试验条件下,本研究使用AMF专性抑制剂苯菌灵,形成有AMF、无AMF对照,探究Epichlo3内生真菌与AMF的互作对宿主羊草(Leymus chinensis)生长的影响。结果表明,在不添加苯菌灵条件下,内生真菌显著增加了AMF侵染率和孢子密度,同时显著增加了羊草的分蘖数、鲜叶数、地上生物量和地下生物量。苯菌灵添加显著降低了AMF的侵染率和孢子密度,且随其添加浓度的增加降低效果更显著;在高浓度苯菌灵添加条件下,感染内生真菌较不感染内生真菌羊草的生长优势消失。Dual infection of host plants by Epichlo3 endophytes and AMF occurs in natural and agricultural ecosystems.Previous studies have focused largely on the effects of endophytes and a single artificial inoculation of AMF on the growth of hosts.However,few studies have investigated the interactive effects between endophytes and AMF on host grasses in natural habitats.In this study,we used benomyl,a specific inhibitor of AMF,manipulating AMF presence or not,to determine the effects of endophytes and/or AMF on the growth of Leymus chinensis.We conducted a pot experiment under two endophyte infection statuses (endophyte-infected,E+;endophytefree,E-) and three benomyl addition levels (B0,control;B1,0.6 g·L;benomyl;B2,1.2g·L;benomyl),using L.chinensis as a model.The results showed that endophyte infection significantly increased AMF colonization rate and spore density,and significantly increased tiller numbers,fresh leaf numbers,shoot and root biomass of L.chinensis.The colonization rate and spore density of AMF were significantly reduced by benomyl,especially under the B2 treatment,and the growth advantage of E+relative to E-L.chinensis disappeared under the B2 treatment.

关 键 词：Epichlo3内生真菌 丛枝菌根真菌 苯菌灵 羊草 生长 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]