不同剂量燧心胶囊对慢性心力衰竭大鼠TrxR活性及ATP、ADP水平的影响  

Effects of Different Doses of Suixin Capsule on TrxR Activity and ATP and ADP Levels in Rats with Chronic Heart Failure

作　　者：郭殿龙 宫照伟[2] 宁益 葛振忠[3] GUO Dian-long;GONG Zhao-wei;NING Yi(The First People’s Hospital of Xianyang,Xianyang Shaanxi,712000)

机构地区：[1]咸阳市第一人民医院,陕西咸阳712000 [2]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001 [3]无锡市人民医院,江苏无锡214000

出　　处：《医学临床研究》2021年第11期1690-1692,1696,共4页Journal of Clinical Research

摘　　要：【目的】探讨燧心胶囊不同剂量对慢性心力衰竭大鼠硫氧还蛋白氧化还原酶(TrxR)活性及三磷酸腺苷(ATP)、二磷酸腺苷(ADP)水平的影响。【方法】选用SPF、健康雄性SD大鼠44只,依据随机数字表法将其分为对照组、西药组、小剂量组、大剂量组,各11只。其中对照组大鼠于正常环境下以动物饲料喂养,西药组、小剂量组、大剂量组通过结扎冠状动脉左前降支的方式建立慢性心力衰竭模型。模型建立成功后饲养8周,至第8周后西药组使用卡托普利灌胃,小剂量组使用燧心胶囊20 g/kg灌胃,大剂量组使用燧心胶囊40g/kg灌胃,三组大鼠每日灌胃1次,连续12周。比较四组大鼠左室舒张末期内径(LVDD)、左室收缩末期内径(LVSD)、左室后壁厚度(LVP-WT)、室间隔厚度(IVST)、左心室射血分数(LVEF),比较四组大鼠心肌组织三磷酸腺苷(ATP)、二磷酸腺苷(ADP)水平肝脏组织硫氧还蛋白酶(Trx)、硫氧还蛋白还原酶(TrxR)水平。【结果】对照组、大剂量组LVEDD、LVEDS、LVP-WT、IVST低于小剂量组、西药组,对照组、大剂量组LVEF水平高于小剂量组、西药组,差异均有统计学意义(P<0.05)。对照组、大剂量组心肌组织ATP、ADP含量及肝脏Trx、TrxR水平高于小剂量组、西药组,差异均有统计学意义(P<0.05)。【结论】燧心胶囊可用于慢性心力衰竭大鼠的治疗,可较好改善大鼠心功能,同时还可改善Trx系统及心肌细胞能量。【Objective】To investigate the effects of different doses of Suixin Capsule on the activity of thiore-doxin oxidoreductase(TrxR)and the levels of adenosine triphosphate(ATP)and adenosine diphosphate(ADP)in rats with chronic heart failure.【Methods】A total of 44 SPF and healthy male SD rats was randomly divided into control group,western medicine group,low-dose group and high-dose group,with 11 rats in each group.The rats in the control group were fed with animal feed under normal environment.The models of chronic heart failure were established by ligating the left anterior descending coronary artery in the western medicine group,low-dose group and high-dose group.After the successful establishment of the model,the rats were fed for 8 weeks.After the 8th week,the western medicine group was fed with captopril,the low-dose group was fed with Suixin capsule 20g/kg,the high-dose group was fed with Suixin capsule 40g/kg,and the three groups were fed once a day for 12 weeks.The left ventricular end diastolic diameter(LVDD),left ventricular end systolic diameter(LVSD),left ventricular posterior wall thickness(LVP WT),ventricular septal thickness(IVST)and left ventricular ejection fraction(LVEF)were compared.The levels of adenosine triphosphate(ATP)and adenosine diphosphate(ADP)in myocardial tissue and thioredoxin reductase(TRx)and thioredoxin reductase(TrxR)in liver tissue were compared Level.【Results】LVED,LVEDS,LVP-WT and IVST in the control group and high-dose group were lower than those in the low-dose group and Western medicine group,and LVEF levels in the control group and the high-dose group were higher than those in the low-dose group and the Western medicine group(P<0.05).The contents of ATP and ADP in myocardial tissue and the levels of TRx and TrxR in liver in the control group and high-dose group were significantly higher than those in the low-dose group and the Western medicine group(P<0.05).【Conclusion】Suixin capsule can be used to treat rats with chronic heart failure,improve cardiac function,

关 键 词：心力衰竭/中药疗法 慢性病 胶囊/治疗应用 心血管药物(中药) 大鼠 

分 类 号：R259.414[医药卫生—中西医结合]