lncRNA MALAT1在正常妊娠和子痫前期患者中的表达差异及临床意义  

Differential expression of lncRNA MALAT1 in normal pregnancy and preeclampsia and its clinical significance

作　　者：张冬梅[1] 杨丽丽 李铮 张会敏[1] ZHANG Dongmei;YANG Lili;LI Zheng;ZHANG Huimin(Department of Obstetrics,Tangshan Municipal Maternity and Child Health Hospital,Hebei,Tangshan 063000,China)

机构地区：[1]河北省唐山市妇幼保健院产科,河北唐山063000

出　　处：《中国妇幼健康研究》2021年第11期1589-1594,共6页Chinese Journal of Woman and Child Health Research

基　　金：河北省医学科学研究课题(20191544)。

摘　　要：目的探讨长链非编码RNA(lncRNA)肺腺癌转移相关转录产物-1 (MALAT1)在正常妊娠和子痫前期(PE)患者中的表达差异及其临床意义。方法选取2018年1月至2020年12月于河北省唐山市妇幼保健院建档并分娩的87例PE患者为实验组(轻度组47例、重度组40例),另选取同期同院分娩的48例健康孕妇为对照组,检测不同组孕妇血清中血小板衍生生长因子-B(PDGF-BB)、胎盘生长因子(PLGF)、血管内皮生长因子(VEGF)、人可溶性FMS样酪氨酸激酶1(sFlt-1),以及血清、胎盘组织lncRNA MALAT1的表达。结果 PE组PDGF-BB、sFlt-1水平显著高于对照组,且重度PE患者显著高于轻度PE患者(F值分别为56.483、67.485,P<0.05);PE组PLGF、VEGF水平显著低于对照组,且重度PE患者显著低于轻度PE患者(F值分别为102.384、19.382,P<0.05);与对照组相比,PE患者血清及胎盘组织中lncRNA MALAT1表达显著降低,且重度PE患者显著低于轻度PE患者(F值分别为6.586、11.347,P<0.05);血清lncRNA MALAT1与血清PDGF-BB、sFlt-1呈负相关,与PLGF、VEGF水平呈正相关(r值分别为-0.789,-0.674、0.567、0.785,P<0.05);lncRNA MALAT1诊断PE的受试者工作特征曲线下面积为0.829(0.755~0.903),敏感度、特异度分别为80.5%、79.2%。结论血清lncRNA MALAT1与PE血清学检测指标有一定相关性,对于PE的早期诊断有一定价值。Objective To investigate difference in expression of long non-coding RNA(lncRNA) metastasis associated lung adenocarcinoma transcript 1(MALAT1) in normal pregnancy and preeclampsia(PE) and its clinical significance.Methods A total of 87 pregnant women with PE who registered and delivered in Tangshan Municipal Maternity and Child Health Hospital in Hebei province from January 2018 to December 2020 were selected as experimental group(47 cases in the mild PE subgroup and 40 cases in the severe PE subgroup),and 48 healthy pregnant women who delivered in the same hospital in the same period were selected as the control group.Serum levels of platelet-derived growth factor-B(PDGF-BB),placental growth factor(PLGF),vascular endothelial growth factor(VEGF),human soluble fms-like tyrosine kinase 1(sFlt-1),and expression of lncRNA MALAT1 in the serum and the placental tissues of the pregnant women in the two groups were detected.Results The serum levels of PDGF-BB and sFlt-1 of the prgnant women in the PE group were significantly higher than those in the control group, and those of the pregnant women in the severe PE subgroup were significantly higher than those in the mild PE subgroup(F=56.483 and 67.485 respectively, both P<0.05).While the serum levels of PLGF and VEGF of the pregnant women in the PE group were significantly lower than those in the control group, and those in the severe PE subgroup were significantly lower than those in the mild PE subgroup(F=102.384 and 19.382 respectively, both P<0.05).Compared with the control group, the expressions of lncRNA MALAT1 in the serum and the placental tissues of PE patients were significantly decreased, and the expression of lncRNA MALAT1 in the severe PE subgroup was significantly lower than that in the mild PE subgroup(F=6.586 and 11.347,both P<0.05).Serum lncRNA MALAT1 was negatively correlated with serum PDGF-BB and sFlt-1,but it was positively correlated with serum PLGF and VEGF(r=-0.789,-0.674,0.567 and 0.785 respectively, all P<0.05).The area under ROC curve of lncRNA

关 键 词：子痫前期 长链非编码RNA 肺腺癌转移相关转录产物-1 受试者工作特征曲线 

分 类 号：R714.2[医药卫生—妇产科学]