纹党参安全性毒理学评价  

Safety toxicological evaluation of Wen Radix Codonopsis

作　　者：许小红[1] 王炯蓉[1] 张东城 冯文燕 宋伟忠 Xu Xiaohong;Wang Jiongrong;Zhang Dongcheng;Feng Wenyan;Song Weizhong(Gansu Provincial Center for Disease Control and Prevention,Lanzhou 730000,China)

机构地区：[1]甘肃省疾病预防控制中心,兰州730000

出　　处：《卫生研究》2021年第6期1012-1018,共7页Journal of Hygiene Research

基　　金：甘肃省中医药管理局科研课题(No.GZKLP-2016-3);兰州市人才创新创业项目(No.2020-RC-26)。

摘　　要：目的对纹党参进行食用安全性毒理学评价。方法参考食品安全国家标准(GB 15193.3—2014、GB 15193.4—2014、GB 15193.5—2014、GB 15193.8—2014、GB 15193.13—2015),对纹党参进行急性经口毒性试验、三项遗传毒性试验(细菌回复突变试验、哺乳动物红细胞微核试验及小鼠精母细胞染色体畸变试验)和亚慢性毒性试验。结果纹党参对昆明小鼠LD_(50)>38.72 g/kg,依据小鼠急性毒性分级标准评价为实际无毒级。三项遗传毒性试验结果均为阴性,未见明显遗传毒性。90天经口毒性试验各组动物总体生长状况良好,各试验组检验指标结果与阴性对照组比较差异无统计学意义,且均在本实验室正常值范围内;大体解剖未见异常,未发现与受试物相关的病理组织学改变和特异性损伤表现。结论纹党参在该条件下未见明显急性经口毒性、遗传毒性以及亚慢性毒性作用。OBJECTIVE To evaluate the toxicological safety of Wen Radix Codonopsis.METHODS According to the national standards of food safety(GB 15193.3—2014,GB 15193.4—2014,GB 15193.5—2014,GB 15193.8—2014,GB 15193.13—2015),acute oral toxicity test,three genetic toxicity tests(including bacterial recovery mutation test,mammalian erythrocyte micronucleus test and mouse spermatocyte chromosome aberration test)and subchronic toxicity test were conducted in this study.RESULTS The LD_(50)of Wen Radix Codonopsis to KM mouse was more than 38.72 g/kg,which was actually non-toxic according to the acute toxicity grading standard of mouse.The results of three genetic toxicity tests were negative and no obvious genotoxicity was observed.90-day oral toxicity test showed that the overall growth condition of the rats in each group was good,and the test index result of each test group showed no statistical significance compared with the negative control group,and all of them were within the normal range in our laboratory.No abnormality was observed in gross anatomy,and no histopathological changes and specific lesions associated with the test substances were found.CONCLUSION No obvious acute oral toxicity,genetic toxicity and subchronic toxicity were observed in Wen Radix Codonopsis under the present conditions.

关 键 词：纹党参 毒理学 安全性评价 

分 类 号：R994.4[医药卫生—毒理学]