柠檬鳞伞生物学特性与驯化栽培  

Biological Characteristics and Artificial Cultivation of Pholiota limonella

作　　者：蔡婧[1] 李世华[1] 李军[1] 李扬 罗义 刘杰[1] 徐章逸[2] 王锋尖 CAI Jing;LI Shihua;LI Jun;LI Yang;LUO Yi;LIU Jie;XU Zhangyi;WANG Fengjian(Shiyan Academy of Agricultural Sciences,Shiyan 442000,Hubei,China;Huazhong Agricultural University,Wuhan 430070,Hubei,China;College of Chemistry and Environmental Engineering,Hanjiang Normal University,Shiyan 442000,Hubei,China)

机构地区：[1]十堰市农业科学院,湖北十堰442000 [2]华中农业大学,湖北武汉430070 [3]汉江师范学院化学与环境工程学院,湖北十堰442000

出　　处：《食用菌学报》2021年第6期61-67,共7页Acta Edulis Fungi

基　　金：湖北省中央引导地方科技发展专项(2019ZYYD039);现代农业产业技术体系建设专项(CARS-20);湖北省食用菌产业技术体系(2021-2024)综合试验站项目(HBHZDZB-2021-023);湖北省生物多样性本底调查观测评估项目。

摘　　要：对采集自湖北省十堰市房县地区的柠檬鳞伞(Pholiota limonella)子实体进行组织分离,并对获得的菌株进行分子鉴定、生物学特性及驯化栽培研究。结果表明:在实验范围内,柠檬鳞伞菌丝生长的最适温度为25℃,最适pH为7.0,最适碳源为麦芽糖和蔗糖,最适氮源为硝酸钾。使用木屑为主要培养料栽培柠檬鳞伞,菌袋接种后于(25±1)℃、黑暗条件下培养,约60 d后菌丝满袋;出菇时的环境温度为10~22℃,相对湿度>90%,光照0~125 lx,开袋后25~30 d形成原基,35~40 d即可采收;平均生物学效率为24.01%。A wild Pholiota limonella fruiting body collected from Fangxian area of Hubei Province was subjected to tissue separation and then identified by molecular markers,studied for biological characteristics and domestication.The results showed that the optimal temperature,pH,carbon source and nitrogen source for mycelial growth were 25℃and 7.0,maltose/sucrose and potassium nitrate,respectively.Using saw dust as the major cultivation substrate material,mycelia fully filled a cultivation bag after cultivation in darkness at(25±1)℃for 60 d.During fruiting body development,the appropriate environmental temperature and illumination condition were 10-22℃and 0-125 lx,respectively.About 25-30 d after bag opening,primordia started to appear and fruiting bodies were harvested around 35-40 d.The average biological efficiency was 24.01%.

关 键 词：柠檬鳞伞 菌丝生长 栽培 

分 类 号：S646[农业科学—蔬菜学]