血浆miR-146a、miR-223水平与儿童急性淋巴细胞白血病预后的相关性  

Correlation of Plasma MiR-146a,MiR-223 Levels with the Prognosis of Childhood with Acute Lymphoblastic Leukemia

作　　者：胡晓燕 尹姣姣 晁荣[1] 朱生东[1] 邓伟[1] 潘铭 HU Xiao-Yan;YIN Jiao-Jiao;CHAO Rong;ZHU Sheng-Dong;DENG Wei;PAN Ming(Department of Pediatric General Medicine,Gansu Maternal and Child Health Hospital,Lanzhou 730050,Gansu Province,China;Department of Laboratory Medicine,Gansu Maternal and Child Health Hospital,Lanzhou 730050,Gansu Province,China;Department of Hematology,Winvei Peopled Hospital,Wuwei 733000,Gansu Province,China)

机构地区：[1]甘肃省妇幼保健院小儿综合内科,甘肃兰州730050 [2]甘肃省妇幼保健院检验科,甘肃兰州730050 [3]武威市人民医院血液科,甘肃武威733000

出　　处：《中国实验血液学杂志》2021年第6期1727-1732,共6页Journal of Experimental Hematology

基　　金：甘肃省自然科学基金(1606RJZH153)。

摘　　要：目的:观察血浆微小RNA(miR)-146a、miR-223在儿童急性淋巴细胞白血病(ALL)患儿中的表达,并分析二者与儿童ALL预后的关系。方法:选取2015年1月-2017年12月本院收治的儿童ALL患儿100例,患儿均根据中国儿童白血病协作组(CCLG)-ALL-2008方案中的标准依据不同危险度在本院进行规范化治疗,并获得随访结果,随访时间≥36个月,随访时间截至2021年3月,以患儿复发及死亡作为预后观察指标;入院时调查员询问并记录患儿基线资料,检测入院时血浆miR-146a、miR-223水平,并分析二者与儿童ALL预后的关系。结果:随访期间,4例患儿死亡,18例患儿复发,即预后不良组患儿22例(22.00%);预后不良组患儿入院时血浆miR-146a水平高于预后良好组,血浆miR-223水平低于预后良好组,差异有统计学意义(P<0.05);回归分析结果显示,入院时血浆miR-146a过表达、miR-223低表达可能与儿童ALL患儿预后不良有关,可能作为患儿预后不良的风险因子(P<0.05);绘制ROC曲线,入院时血浆miR-146a、miR-223单独及联合预测儿童ALL患儿预后不良风险的AUC>0.80,均有一定预测价值;相关性检验结果显示,入院时血浆miR-146a、miR-223水平之间呈负相关(r=-0.239,P=0.016)。结论:血浆miR-146a在儿童ALL中过表达,miR-223在儿童ALL中低表达,二者异常表达与儿童ALL患儿预后存在一定联系。Objective: To observe the expression of plasma microRNA( miR)-146 a and miR-223 in children with acute lymphoblastic leukemia( ALL),so as to analyze the relationship between the two factors and the prognosis of children with ALL. Methods: 100 children with ALL treated in the hospital from January 2015 to December 2017 were selected,according to the standard of Chinese Children’s Leukemia Group( CCLG)-ALL-2008 program,the children were performed standardized treatment in our hospital according to different risk degree,the follow-up results were obtained,the follow-up time was ≥36 months,and the follow-up time was till to March 2021,the recurrence and mortality of the children were used as prognostic indicators;the baseline data of the children at admission were inquired and recorded,the plasma miR-146 a and miR-223 levels were analyzed at admission,and their correlation with the prognosis of children with ALL was analyzed. Results: During the follow-up period,4 cases of children died while 18 cases recurred,which means 22( 22. 00%) children showed the poor prognosis;the plasma miR-146 a level of the children in poor prognosis group at admission was higher than those in good prognosis group,while the plasma miR-223 level was lower than those in good prognosis group,the differences showed statistically significantly( P< 0.05);the results of regression analysis showed that the over expression of plasma miR-146 a and low expression of plasma miR-223 at admission might be associated with poor prognosis in ALL children,and might be a risk factor for poor prognosis in children( P<0.05);the ROC curve showed that the AUC of plasma miR-146 a and miR-223 at admission alone or in combination showed the predictive value for the risk of poor prognosis in children with ALL( AUC >0.80);the results of correlation test showed that there was a negative correlation of plasma miR-146 a with miR-223 levels at admission( r =-0.239,P= 0.016). Conclusion: Plasma miR-146 a is overexpressed and miR-223 is low-expressed in children with

关 键 词：急性淋巴细胞白血病 儿童 微小RNA-146a 微小RNA-223 预后 相关性 

分 类 号：K733.71[历史地理—历史学]