三种证型对哮喘小鼠特异性/非特异性免疫功能及气道炎症的相关性研究  

Correlation study of three types of syndromes on specific/nonspecific immune function and airway inflammation in asthmatic mice

作　　者：孙雅妮 陈诗 唐寒 葛月宾[1] SUN Yani;CHEN Shi;TANG Han;GE Yuebin(School of Pharmaceutical Science, South-central University for Nationalities, Wuhan 430074, China)

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2021年第6期597-604,共8页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：湖北省卫生健康委员会中医药科研面上项目(ZY2021M091);中央高校基本科研业务费专项资金资助项目(CZY20049)。

摘　　要：采用病证结合方法,构建三种证型肺气虚、脾气虚、肺脾气虚哮喘小鼠模型,研究三种证型对哮喘小鼠特异性/非特异性免疫功能及气道炎症的影响及关联性.与空白对照组相比,脾气虚、肺脾气虚哮喘小鼠T·B淋巴细胞增殖功能降低、T细胞亚型CD3+、CD4+占比减少、IgG、IgA、IgM表达水平下降(P<0.05);且三种证型哮喘小鼠BALF中各类炎性细胞计数、分泌的细胞因子IL-4、IL-5、IL-13含量增多,且肺脾气虚哮喘组气道阻力上升至2倍(P<0.05).模型组之间相比,肺脾气虚哮喘组较单纯哮喘组和肺气虚哮喘组T淋巴细胞增殖功能降低,T细胞亚型CD3+、CD4+比例下降,体液免疫指标IgA、IgM含量下降明显,且肺脾气虚哮喘小鼠BALF中嗜酸性粒细胞增加、血清中OVA-IgE浓度高于单因素证候模型组(P<0.05).结果表明:三种证型对哮喘小鼠特异性/非特异性免疫功能及气道炎症有影响且具有一定关联性.The combination of disease and syndrome was used to construct three syndromes of asthma mouse models to study the effects of the three syndromes including spleen-qi deficiency asthma,lung-qi deficiency asthma and lung-spleen qi deficiency asthma.Compared with the blank control group,immune organ index,T·B lymphocyte proliferation,T cell subtype CD3+,CD4+proportion,humoral immune index IgG,IgA,IgM content in the asthma group with deficiency of spleen-qi and asthma group with deficiency of lung spleen qi decreased(P<0.05).IL-4,IL-5,and IL-13 in the BALF of the three types of asthma groups increased,and the airway resistance in the asthma group with the deficiency of lung-spleen-qi increased to 2 times of the blank group(P<0.05).Compared with the model groups,the deficiency of lung-spleen qi asthma group was lower than the simple asthma group and the lung-qi deficiency asthma group in terms of immune organ index,T lymphocyte proliferation,T cell subtype CD3+,CD4+ratio,humoral immune index content,and deficiency of lung-spleen qi asthma group.The concentrations of eosinophils and serum OVA-IgE in BALF were higher than those in the single-factor syndrome model group(P<0.05).The results show that the three types of syndromes have effects on specific/non-specific immune function and airway inflammation in asthmatic mice and have certain correlations.

关 键 词：支气管哮喘 肺脾气虚证 免疫功能 气道炎症 气道阻力 

分 类 号：R285.5[医药卫生—中药学]