Distinct immune signatures discriminate between asymptomatic and presymptomatic SARS-CoV-2^(pos)subjects  

作　　者：Shanhe Yu Caixia Di Shijun Chen Mingquan Guo Jiayang Yan Zhaoqin Zhu Li Liu Ruixue Feng Yinyin Xie Ruihong Zhang Juan Chen Mengxi Wang Dong Wei Hai Fang Tong Yin Jinyan Huang Saijuan Chen Hongzhou Lu Jiang Zhu Jieming Qu 

机构地区：[1]Shanghai Institute of Hematology,State Key Laboratory of Medical Genomics,National Research Center for Translational Medicine at Shanghai,Collaborative Innovation Center of Hematology,Ruijin Hospital affiliated to Shanghai Jiao-Tong University School of Medicine,Shanghai,China [2]Department of Respiratory and Critical Care Medicine,Ruijin Hospital,Institute of Respiratory Diseases,School of Medicine,Shanghai Jiao-Tong University,Shanghai,China [3]Key Laboratory of Emergency Prevention,Diagnosis and Treatment of Respiratory Infectious Diseases,Shanghai,China [4]Department of Laboratory Medicine,Shanghai Public Health Clinical Center,Fudan University,Shanghai,China [5]Department of Infectious Diseases and Immunology,Shanghai Public Health Clinical Center,Fudan University,Shanghai,China [6]Department of Infectious Disease,Research Laboratory of Clinical Virology,Ruijin Hospital,School of Medicine,Shanghai Jiao-Tong University,Shanghai,China [7]National Research Center for Translational Medicine at Shanghai,Shanghai,China

出　　处：《Cell Research》2021年第11期1148-1162,共15页细胞研究（英文版）

基　　金：This work was supported by Key Laboratory of Emergency Prevention,Diagnosis and Treatment of Respiratory Infectious Diseases in Shanghai(20dz2261100);the National Major Scientific and Technological Special Project(2018ZX09101001);Cultivation Project of Shanghai Major Infectious Disease Research Base(20dz2210500);the National Natural Science Foundation of China(NSFC)(81430002,81770206,81900148);the National Innovative Research Team of High-level Local Universities in Shanghai,Shanghai Collaborative Innovation Program on Regenerative Medicine and Stem Cell Research(2019CXJQ01);the National Research Center for Translational Medicine at Shanghai,RiHjin Hospital Affiliated to Shanghai Jiao-Tong University School of Medicine[NRCTM(SH)-2019-02];the National Key R&D Program of China(2018YFA0107802);Shanghai Rising-Star Program(20QC1400100);Samuel Waxman Cancer Research Foundation,Novo Nordisk Hemophilia Research Fund in China and Emergency Project of Shanghai Science and Technology Commission(20411950502).

摘　　要：Increasing numbers of SARS-CoV-2-positive(SARS-CoV-2^(pos))subjects are detected at silent SARS-CoV-2 infection stage(SSIS).Yet,SSIS represents a poorly examined time-window wherein unknown immunity patterns may contribute to the fate determination towards persistently asymptomatic or overt disease.Here,we retrieved blood samples from 19 asymptomatic and 12 presymptomatic SARS-COV-2^(pos)subjects,47 age/gender-matched patients with mild or moderate COVID-19 and 27 normal subjects,and interrogated them with combined assays of 44-plex CyTOF,RNA-seq and Olink.Notably,both asymptomatic and presymptomatic subjects exhibited numerous readily detectable immunological alterations,while certain parameters including more severely decreased frequencies of CD107a^(low)classical monocytes,intermediate monocytes,non-classical monocytes and CD62L^(hi)CD8^(+)Tnaive cells,reduced plasma STC1 level but an increased frequency of CD4^(+)NKT cells combined to distinguish the latter.Intercorrelation analyses revealed a particular presymptomatic immunotype mainly manifesting as monocytic overactivation and differentiation blockage,a likely lymphocyte exhaustion and immunosuppression,yielding mechanistic insights into SSIS fate determination,which could potentially improve 5ARS-CoV-2 management.

关 键 词：ASYMPTOMATIC immunity alterations 

分 类 号：R392[医药卫生—免疫学]