钩毛茜草正丁醇部位化学成分的研究  

Chemical constituents from the n-butanol fraction of Rubia oncotricha

作　　者：朱敏汇 杨贵香 郑林[2] 汪洋 马雪[1] 林昌虎[1] 李勇军[1] ZHU Min-hui;YANG Gui-xiang;ZHENG Lin;WANG Yang;MA Xue;LIN Chang-hu;LI Yong-jun(Engneering Research Center for the Development and Application of Ethnic Medicine and TCM/State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,China;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University,Guiyang 550004,China;School of Pharmacy,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学民族药与中药开发应用教育部工程研究中心/省部共建药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学贵州省药物制剂重点实验室,贵州贵阳550004 [3]贵州医科大学药学院,贵州贵阳550004

出　　处：《中成药》2021年第12期3367-3372,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(U1812403);中央引导地方科技专项项目(黔科中引地[2018]4006);贵州省科学技术厅人才项目(黔科合平台人才[2016]5677)。

摘　　要：目的研究钩毛茜草Rubia oncotricha Hand.-Mazz.正丁醇部位的化学成分。方法钩毛茜草70%乙醇提取物正丁醇部位采用硅胶、Sephadex LH-20、制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到15个化合物,分别鉴定为落叶松脂醇-4′-O-β-D-葡萄糖苷(1)、11-demethoxy-11-ethoxydaphylloside(2)、鸡屎藤酸甲酯(3)、10-acetylborreriagenin(4)、去乙酰车叶草苷酸乙酯(5)、(-)-(7S,8R)-4,9,9′-trihydroxy-3′,5,-dimethoxy-4′,7-epoxy-8,3′-neoligan-9-O-[α-L-rhamnopyranosyl(1→6)]-β-D-glucopyranoside(6)、(7S,8R)-脱氢双松柏醇-9′-β-葡萄糖苷(7)、落叶松脂醇-4-O-β-D-葡萄糖苷(8)、没食子酸乙酯(9)、车叶草酸(10)、车叶草苷(11)、腺苷(12)、β-胡萝卜苷(13)、没食子酸(14)、齐墩果酸(15)。结论化合物1~9为首次从茜草属植物中分离得到,化合物1~14为首次从该植物中分离得到。AIM To study the chemical constituents from the n-butanol fraction of Rubia oncotricha Hand.-Mazz..METHODS The n-butanol fraction of 70%ethanol extract from R.oncotricha was isolated and purified by silica,Sephadex LH-20 and preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Fifteen compounds were isolated and identified as lariciresinol-4′-O-β-D-glucopyranoside(1),11-demethoxy-11-ethoxydaphylloside(2),paederosidic acid methyl ester(3),10-acetylborreriagenin(4),deacetylasoerulosidic acid ethyl ester(5),(-)-(7S,8R)-4,9,9′-trihydroxy-3′,5,-dimethoxy-4′,7-epoxy-8,3′-neoligan-9-O-[α-L-rhamnopyranosyl(1→6)]-β-D-glucopyranoside(6),(7S,8R)-dehydrodiconiferyl alcohol-9′-β-glucopyranoside(7),lariciresinol-4-O-β-D-glucopyranoside(8),ethyl-3,4,5-trihydroxybenzoate(9),asperulosidic acid(10),asperuloside(11),adenosine(12),daucosterol(13),gallic acid(14),oleanolic acid(15).CONCLUSION Compounds 1-9 are isolated from genus Rubia for the first time.Compounds 1-14 are first isolated from this plant.

关 键 词：钩毛茜草 正丁醇部位 化学成分 分离鉴定 

分 类 号：R284.1[医药卫生—中药学]