山蜡梅叶酚性成分的研究  

Phenolic constituents from the leaves of Chimonanthus nitens

作　　者：郭娜 田燕元 舒任庚 刘嘉丽 GUO Na;TIAN Yan-yuan;SHU Ren-geng;LIU Jia-li(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学药学院,江西南昌330004

出　　处：《中成药》2021年第12期3378-3382,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81360631);江西省重点研发计划项目(20203BBGL73218)。

摘　　要：目的研究山蜡梅Chimonanthus nitens Oliv.叶中酚性成分。方法山蜡梅叶90%乙醇提取物采用硅胶、ODS、Sephadex LH-20、AB-8大孔吸附树脂及半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到13个酚性化合物,分别鉴定为3,4-二羟基苯甲酸甲酯(1)、对羟基苯甲酸甲酯(2)、对羟基苯乙酸甲酯(3)、对苯二酚(4)、对羟基苯甲酸乙酯(5)、ficusol(6)、methyl p-hydroxy-trans-cinnamate(7)、trans-cinnamic acid(8)、trans-p-hydroxyl ethyl cinnamate(9)、avenalumic acid methyl ester(10)、丁香酯素(11)、决明顺反二聚苯丙素(12)、东莨菪素(13)。结论化合物1、3、6~7、9~12为首次从该属植物中分离得到,化合物1、3~4、6~12为首次从该植物中分离得到。AIM To study the phenolic constituents from the leaves of Chimonanthus nitens Oliv..METHODS The 90%ethanol extract from the leaves of C.nitens was isolated and purified by silica、ODS、Sephadex LH-20、AB-8 macroporous resin,and semi-reparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Thirteen phenolic compounds were isolated and identified as 3,4-dihydroxybenzoic acid methyl ester(1),methylβ-hydroxybenzoate(2),p-hydroxyphenylacetic acid methyl ester(3),p-dihydroxybenzene(4),p-hydroxybenzoic acid ethyl ester(5),ficusol(6),methyl p-hydroxy-trans-cinnamate(7),trans-cinnamic acid(8),trans-p-hydroxyl ethyl cinnamate(9),avenalumic acid methyl ester(10),(+)-syringaresinol(11),cassia cis-trans diphenylpropanoid(12),scopoletin(13).CONCLUSION Compounds 1,3,6-7,9-12 are isolated from this genus Chimonanthus for the first time,and compounds 1,3-4,6-12 are isolated from this plant for the first time.

关 键 词：山蜡梅 叶 酚性成分 分离鉴定 

分 类 号：R284.1[医药卫生—中药学]