CIM法应用于鲍曼不动杆菌和铜绿假单胞菌耐碳青霉烯类检测的价值  

The Value of CIM Method in the Detection of Carbapenem Resistance of Acinetobacter Baumannii and Pseudomonas Aeruginosa

作　　者：肖正勤[1] 李江[1] XIAO Zheng-qin;LI Jiang(Dongguan Chang'an Hospital,Guangdong Dongguan 523843)

机构地区：[1]东莞市长安医院,广东东莞523843

出　　处：《深圳中西医结合杂志》2021年第18期112-114,共3页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:探讨改良Carba NP法和碳青霉烯酶失活(CIM)法应用于鲍曼不动杆菌和铜绿假单胞菌耐碳青霉烯类抗菌药物快速检测中的价值。方法:纳入东莞市长安医院2020年3月至2021年3月期间临床分离的88株铜绿假单胞菌及88株鲍曼不动杆菌为研究对象,所有菌株均采用改良Carba NP法和CIM法检测,后以基因检测作为金标准,观察两种方法的检测价值(灵敏度、特异度、阴性预测值与阳性预测值)。结果:铜绿假单胞菌通过CIM法检测的灵敏度(96.15%)、阴性预测值(75.00%)均高于改良Carba NP法(84.62%、29.41%),差异均具有统计学意义(P<0.05);特异度(90.00%)、阳性预测值(98.68%)与改良Carba NP法(50.00%、92.96%)比较,差异均无统计学意义(P>0.05);鲍曼不动杆菌通过CIM法检测的灵敏度(90.67%)、特异度(92.31%)、阴性预测值(63.16%)及(98.55%)均高于改良Carba NP法(78.67%、53.85%、30.43%、90.77%),差异均具有统计学意义(P<0.05)。结论:CIM法应用于鲍曼不动杆菌和铜绿假单胞菌耐碳青霉烯类抗菌药物快速检测的价值比改良Carba NP法更高。Objective To explore the value of modified Carba NP method and carbapenemase inactivation(CIM)method for rapid detection of carbapenem-resistant antibacterial drugs in Acinetobacter baumannii and Pseudomonas aeruginosa.Methods The 88 strains of Pseudomonas aeruginosa and 88 strains of Acinetobacter baumannii clinically isolated from Dongguan Chang'an Hospital from March 2020 to March 2021 were included as the research objects.All the strains used the modified Carba NP method and CIM method After detection,genetic testing was used as the gold standard to observe the detection value of the two methods(sensitivity,specificity,negative predictive value and positive predictive value).Results The detection sensitivity(96.15%)and negative predictive value(75.00%)of Pseudomonas aeruginosa by the CIM method were higher than those of the modified Carba NP method(84.62%,29.41%),and the differences were statistically significant(P<0.05);Compared with the specificity(90.00%),positive predictive value(98.68%)and the modified Carba NP method(50.00%,92.96%),the difference was not statistically significant(P>0.05);acinetobacter baumannii was detected by the CIM method Sensitivity(90.67%),specificity(92.31%),negative predictive value(63.16%)and(98.55%)are all higher than the modified Carba NP method(78.67%,53.85%,30.43%,90.77%),and the differences are statistically significant Academic significance(P<0.05).Conclusion The CIM method is more valuable than the modified Carba NP method for the rapid detection of carbapenem-resistant antibacterial drugs in Acinetobacter baumannii and Pseudomonas aeruginosa.

关 键 词：耐碳青霉烯类抗菌药物快速检测 铜绿假单胞菌 鲍曼不动杆菌 碳青霉烯酶失活法 改良Carba NP法 

分 类 号：R446.5[医药卫生—诊断学]