甘草愈伤组织诱导及带芽茎段丛生芽增殖条件优化  

Optimization on the Callus Induction and Proliferation of Cluster Budsfrom Stem Segments with Buds of Glycyrrhiza Uralensis

作　　者：李伟 张东向[1] 刘丽杰[1] 吕晴 任艳波[1] LI Wei;ZHANG Dong-xiang;LIU Li-jie;LYU Qing;REN Yan-bo(College of Life Sciences and Agriculture and Forestry,Qiqihar University/Heilongjiang Key Laboratory of Resistance Gene Engineering and Biodiversity Protection in Cold Areas,Qiqihar,Heilongjiang 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院/抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《福建农业科技》2021年第10期27-33,共7页Fujian Agricultural Science and Technology

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(YSTSXK201887);齐齐哈尔市科技攻关项目(SFGG201575);齐齐哈尔大学研究生创新项目(YJSCX2019044)。

摘　　要：为筛选适于甘草愈伤组织诱导及带芽茎段丛生芽增殖的最佳激素配比,选取萌发能力最强的甘草种子作为试验材料,获得无菌苗,取其下胚轴和带芽茎段为外植体,在不同激素配比下诱导愈伤组织,统计愈伤组织的生长状况、颜色状态、诱导率和出愈率等指标,并研究不同激素对甘草带芽茎段丛生芽增殖的影响,统计带芽茎段丛生芽株高、节间距、真叶数、增殖倍数等指标。结果表明:MS+6 BA 1.0 mg·L^(-1)+2,4 D 2.0 mg·L^(-1)和MS+6 BA 1.0 mg·L^(-1)+NAA 2.0 mg·L^(-1)有利于下胚轴愈伤组织的诱导,MS+6 BA 0.2~0.6 mg·L^(-1)有利于带芽茎段愈伤组织的形成;MS+6 BA 1.8 mg·L^(-1)对带芽茎段的生长最为有利,MS+6 BA 1.0~2.2 mg·L^(-1)有利于带芽茎段丛生芽的增殖,随着6 BA浓度的升高,甘草带芽茎段丛生芽的增殖倍数也随之增加。In order to screen the best hormone ratio for callus induction and the proliferation of cluster buds from the stem segments with buds of Glycyrrhiza uralensis,the seeds of Glycyrrhiza uralensis with the strongest germination ability were selected as the experimental materials to obtain the aseptic seedlings.The hypocotyls and stem segments with buds were taken as the explants to induce the callus under different hormone ratios,and the growth status,color status,induction rate and healing rate of callus were counted.Then,the effects of different hormones on the proliferation of cluster buds from the stem segments with buds of Glycyrrhiza uralensis were studied.And the indicators such as plant height,node spacing,number of true leaves and proliferation multiple of cluster buds from the stem segments with buds were counted.The results showed that MS+6 BA 1.0 mg·L^(-1)+2,4 D 2.0 mg·L^(-1) and MS+6 BA 1.0 mg·L^(-1)+NAA 2.0 mg·L^(-1) were conducive to the induction of hypocotyl callus,and MS+6 BA 0.2-0.6 mg·L^(-1) was conducive to the callus formation of the stem segments with buds.MS+6 BA 1.8 mg·L^(-1) was the most beneficial to the growth of the stem segments with buds,and MS+6 BA 1.0-2.2 mg·L^(-1) was conducive to the proliferation of cluster buds from the stem segments with buds.With the increase of 6 BA concentration,the proliferation multiple of cluster buds from the stem segments with buds of Glycyrrhiza uralensis also increased.

关 键 词：甘草 愈伤组织 带芽茎段 丛生芽 激素 

分 类 号：S567.23[农业科学—中草药栽培]