华中枸骨根化学成分研究  

Chemical constituents from roots of Ilex centrochinensis

作　　者：路艳霞 阳航 石妤 李路军[1,2] 胡泽华 邓雪莹 易英韬 孙可可 LU Yan-xia;YANG Hang;SHI Yu;LI Lu-jun;HU Ze-hua;DENG Xue-ying;YI Ying-tao;SUN Ke-ke(National&Local Joint Engineering Research Center of High-throughput Drug Screening technology,State Key Laboratory of Biocatalysis and Enzyme Engineering,Hubei Key Laboratory of Biotechnology of Chinese Traditional Medicine,Hubei University,Wuhan 430062,China;Gansu Herbal Medicine Planting Co.,Ltd.,Lanzhou 730102,China;Medical School,Hubei Minzu University,Enshi 445000,China)

机构地区：[1]药物高通量筛选国家与地方联合工程研究中心,生物催化与酶工程国家重点实验室,中药生物技术湖北省重点实验室,湖北大学,湖北武汉430062 [2]甘肃百草中药材种植有限公司,甘肃兰州730102 [3]湖北民族大学医学部,湖北恩施445000

出　　处：《中草药》2021年第22期6790-6795,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81560703);深圳市基础研究计划自由探索项目(JCYJ20170306171157738);药物高通量筛选技术国家地方联合工程研究中心开放基金(M20181007,M20202003);药学“荆楚卓越人才”协同育人计划大学生创新训练计划项目(M20191002)。

摘　　要：目的研究华中枸骨Ilex centrochinensis根的化学成分。方法运用硅胶、Sephadex LH-20,ODS柱色谱及半制备HPLC等方法进行分离纯化,并通过NMR等波谱技术和理化性质鉴定结构。结果从华中枸骨根中分离得到13个化合物,分别鉴定为11-羰基-ɑ-香树醇-3β-棕榈酸酯(1)、3β-乙酰氧基-28-羟基-12-烯-乌苏烷(2)、儿茶酚(3)、4-乙基儿茶酚(4)、2,4,6-三甲氧基苯酚(5)、3-(4-羟基-3,5-二甲氧基苯基)-1,2-丙二醇(6)、3-(4-羟基-3-甲氧基苯基)-1,2-丙二醇(7)、3,4-二甲氧基苯酚(8)、3,4-二羟基苯丙酸甲酯(9)、3β,19α,23-三羟基-20α(H)-乌苏-12-烯-28-酸3β-O-α-L-吡喃阿拉伯糖(10)、(+)-1-羟基松脂醇-1-O-β-D-葡萄糖苷(11)、3β-O-[α-L-吡喃鼠李糖(1→2)-α-L-吡喃阿拉伯糖]-坡模酸(12)、rotundioic acid(13)。结论所有化合物均为首次从华中枸骨中分离得到,其中4~9为首次从冬青属植物中分离得到。Objective To investigate the chemical constituents from the roots of Ilex centrochinensis.Methods The chemical constituents were isolated and purified by silica gel,Sephadex LH-20,ODS and semi-prepared high performance liquid chromatography and their structures were elucidated on the basis of NMR technique and physicochemical property.Results Thirteen compounds were obtained from the roots of I.centrochinensis,and named as 11-carbonyl-ɑ-amyrin-3β-palmitate(1),3β-acetoxy-28-hydroxy-12-ene-urs(2),catechol(3),4-ethyl catechol(4),2,4,6-trimethoxyphenol(5),3-(4-hydroxy-3,5-dimethoxyphenyl)propane-1,2-diol(6),3-(4-hydroxy-3-methoxyphenyl)propane-1,2-diol(7),3,4-dimethoxyphenol(8),3,4-dihydroxybenzenepropionic acid methyl ester(9),3β,19α,23-trihydroxy-20α(H)-urs-12-en-28-oic acid 3β-O-α-L-arabinopyranoside(10),(+)-1-hydroxypinoresinol-1-O-β-D-glucoside(11),3β-O-[α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranosyl]-pomolic acid(12),rotundioic acid(13).Conclusion All compounds were isolated from I.centrochinensis for the first time,and compounds 4-9 were isolated from Ilex for the first time.

关 键 词：华中枸骨 11-羰基-ɑ-香树醇-3β-棕榈酸酯 3-(4-羟基-3 5-二甲氧基苯基)-1 2-丙二醇 (+)-1-羟基松脂醇-1-O-β-D-葡萄糖苷 

分 类 号：R284.1[医药卫生—中药学]