买麻藤中1个新的含氮芪类化合物  

A new nitrogen-bearing stilbene from Gnetum montanum

作　　者：王万方 仇雪 王飞 姜先俊 魏国柱 李香梅 WANG Wan-fang;QIU Xue;WANG Fei;JIANG Xian-jun;WEI Guo-zhu;LI Xiang-mei(BioBioPha Co.,Ltd.,Kunming 650201,China;Reference Substance Branch,National Engineering Research Center for Modernization of Traditional Chinese Medicine,Kunming 650201,China)

机构地区：[1]云南西力生物技术股份有限公司,云南昆明650201 [2]国家中药现代化工程技术研究中心标准物质分中心,云南昆明650201

出　　处：《中草药》2021年第21期6460-6465,共6页Chinese Traditional and Herbal Drugs

基　　金：国家发改委“大规模综合性化合物库建设”专项基金。

摘　　要：目的研究买麻藤Gnetum montanum藤茎的化学成分。方法利用正相硅胶、Sephadex LH-20凝胶、中压制备液相色谱和半制备HPLC等多种柱色谱技术进行分离纯化,并综合运用ESI-MS、HR-ESI-MS、^(1)H-NMR、^(13)C-NMR、DEPT、^(1)H-^(1)H COSY、HSQC、HMBC等方法鉴定化合物的结构。结果从买麻藤藤茎的95%乙醇提取物中分离得到14个化合物,分别鉴定为(Z)-5-(2,4-二羟基-6-(4-羟基-3-甲氧基苯乙烯基)苯基)吡咯烷-2-酮(1)、银松素单甲醚(2)、3-羟基-5,4′-二甲氧基芪(3)、银松素(4)、买麻藤醇(5)、刚果买麻藤素F(6)、异丹叶大黄素(7)、闭苞买麻藤素(8)、白藜芦醇(9)、顺式-ε-葡萄素(10)、ε-葡萄素(11)、双异食用大黄苷元A(12)、顺式-射干素B(13)和小叶买麻藤素A(14)。结论化合物1为新化合物,命名为顺式买麻藤内酰胺(cis-gnetumamide),化合物3、10、13和14为首次从该植物中分离得到。Objective To study the chemical constituents from the stems of Gnetum montanum.Methods The compounds were isolated and purified by silica gel,Sephadex LH-20 gel column chromatography,preparative MPLC,and semi-preparative HPLC.Their structures were fully determined based on spectroscopic analysis including ESI-MS,HR-ESI-MS,^(1)H-NMR,^(13)C-NMR,DEPT,^(1)H-^(1)H COSY,HSQC and HMBC spectra.Results Fourteen compounds were isolated and identified from 95%ethanol extract of the stems of G.montanum.The structure of 1 was established as(Z)-5-(2,4-dihydroxy-6-(4-hydroxy-3-methoxystyryl)phenyl)pyrrolidin-2-one(1),and the other compounds were identified as pinosylvin monomethyl ether(2),3-hydroxy-5,4′-dimethoxystilbene(3),pinosylvin(4),gnetol(5),gneafricanin F(6),isorhapontigenin(7),gnetulin(8),resveratrol(9),cis-ε-viniferin(10),ε-viniferin(11),bisisorhapontigenin A(12),cis-shegansu B(13)and gnetifolin A(14).Conclusion Compound 1 is a new compound and named cis-gnetumamide.Compounds 3,10,13 and 14 were isolated from the plant for the first time.

关 键 词：买麻藤 含氮芪类 顺式买麻藤内酰胺 白藜芦醇 顺式-射干素B 小叶买麻藤素A 

分 类 号：R284.1[医药卫生—中药学]