UPLC-Q-TOF-MS/MS快速分析六味地黄苷糖片化学成分  

Rapid identification of chemical components in Liuwei dihuang gantang Tablets by UPLC-Q-TOF-MS/MS

作　　者：樊晓荃 付娟[2,3] 胡军华[2,3] 肖伟[2,3] 王振中[1,2,3] FAN Xiao-quan;FU Juan;HU Jun-hua;XIAO Wei;WANG Zhen-zhong(Nanjing University of Chinese Medicine,Nanjing 210000,China;Jangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process,Lianyungang 222001,China)

机构地区：[1]南京中医药大学,江苏南京210000 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中草药》2021年第21期6473-6484,共12页Chinese Traditional and Herbal Drugs

基　　金：重大新药创制科技重大专项(2013ZX09402203)。

摘　　要：目的采用UPLC-Q-TOF-MS/MS技术对六味地黄苷糖片中的化学成分进行分析和鉴定。方法色谱柱为Phenomenex Luna C_(18)(250 mm×4.6 mm,5μm),以乙腈-0.3%甲酸水溶液作为流动相,梯度洗脱,体积流量1 mL/min,柱温35℃,进样量10μL;采用电喷雾离子源,负离子模式下采集数据,根据精确质荷比、二级碎片离子信息,结合对照品和文献数据鉴定六味地黄苷糖片中的化学成分。结果共鉴定和推测出71个化合物,其中25个来自酒萸肉、19个来自熟地黄、31个来自牡丹皮、2个来自泽泻、3个来自茯苓、2个来自山药。其中22个化合物经对照品验证。结论基本明确了六味地黄苷糖片的化学物质基础,为六味地黄苷糖片的质量控制及药效物质基础研究提供了参考。Objective To study the chemical constituents in Liuwei Dihuang gantang tablets(六味地黄糖苷片)by using UPLC-Q-TOF-MS/MS.Methods The chromatographic conditions were as follows:Phenomenex Luna C_(18)(250 mm×4.6 mm,5μm)column with acetonitrile and 0.3%formic acid as mobile phase,gradient elution,flow rate of 1 mL/min,The column temperature was 35 degrees,the injection volume was 10 L;mass spectrometry conditions:The data were collected by electrospray ion source(ESI)under negative ion mode.These components were further analyzed based on the accurate mass ratio,the two level fragment ion information,the reference materials and literature data.Results A total of 71 compounds were identified and speculated,25 of which came from liquor cornus,19 from Rehmannia Radix Praeparata,31 from Mutan Cortex,2 from Alismatis Rhizoma,3 from Poria and 2 from Dioscoreae Rhizoma.Among them,22 compounds were verified by reference substance.Conclusion The results basically clarified the chemical basis of Liuwei Dihuang gantang tablets,and provided a reference for the quality control and pharmacodynamic material basis of Liuwei Dihuang gantang tablets.

关 键 词：六味地黄苷糖片 质谱分析 质量控制 化学成分谱 酒萸肉 熟地黄 牡丹皮 泽泻 茯苓 山药 

分 类 号：R284.1[医药卫生—中药学]