基于盐炙前后成分煎出差异与止泻作用相关性探讨补骨脂丸增效机制  

To explore synergistic mechanism of Buguzhi Pills based on correlation between antidiarrheal effect and difference of ingredients before and after salt processing

作　　者：毋启桐 王金金[1] 杨安南 周宁[1,2] 赵强 李凯 张振凌[1,2] WU Qi-tong;WANG Jin-jin;YANG An-nan;ZHOU Ning;ZHAO Qiang;LI Kai;ZHANG Zhen-ling(Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Research Center for Special Processing Technology of Chinese Medicine,Zhengzhou 450046,China;Center for Evaluation and Inspection of Hubei Medical Products Administration,Wuhan 430071,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南省中药特色炮制技术工程研究中心,河南郑州450046 [3]湖北省药品监督管理局技术审评核查中心,湖北武汉430071

出　　处：《中草药》2021年第21期6493-6500,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81873005);河南省高校科技创新人才支持计划(21HASTIT047);河南省高等学校青年骨干教师培养计划(2018GGJS083);大学生创新创业训练计划项目(201810471002)。

摘　　要：目的探讨盐炙对补骨脂丸中代表性成分煎出的影响,以及成分与止泻药效的相关性。方法采用HPLC-MS法测定补骨脂丸及3种补骨脂丸模拟品水煎液中13种代表性成分的含量差异,并结合不同给药组对脾肾阳虚泄泻模型大鼠的胃肠激素和下丘脑-垂体-肾上腺轴相关指标的影响,采用灰色关联分析法,对代表性成分与止泻药效进行相关性分析,寻找药效贡献较大成分。结果与3种模拟品(模拟I~III号)相比,补骨脂丸水煎液中黄酮类成分如芦丁、异补骨脂二氢黄酮、新补骨脂异黄酮、补骨脂二氢黄酮、补骨脂宁、异补骨脂查耳酮及补骨脂二氢黄酮甲醚的含量较高,挥发油类成分如茴香醛、反式茴香脑及补骨脂酚的含量较低;与模拟II、III号相比,香豆素类成分如补骨脂素及异补骨脂素的含量较低。不同成分对止泻药效的贡献具有差异,黄酮类和挥发油类成分与止泻药效的关联度较高,香豆素类成分(如补骨脂素与异补骨脂素)与止泻药效的关联度较低。结论补骨脂、小茴香盐炙后组成补骨脂丸,能促进黄酮类成分煎出,抑制挥发油类、香豆素类成分煎出,这可能是盐炙增强其止泻作用的关键环节之一。Objective To explore the influence of salt processing on the representative decoction components of Buguzhi Pills(补骨脂丸),and the correlation between the components and the antidiarrhea effect.Methods HPLC-MS was used to determine the differences of 13 representative components in decoction of Buguzhi Pills and three simulants of Buguzhi Pills.Combined with the different experimental groups’results on spleen-kidney yang deficiency diarrhea model rats about their gastrointestinal hormone and related indexes of hypothalamus-pituitary-adrenal axis,then gray correlation analysis method was used to analyze the correlation between the ingredients and the antidiarrhea effects to determine major contributors in efficacy.Results Compared with the three simulants,the decoction of Buguzhi pills was higher in flavonoids such as rutinum,isobavachin,neobavaisoflavone,bavachin,corylin,isobavachalcone and bavachinin,while it was lower in volatile oils,for instance,anisaldehyde,trans-anethol and bakuchiol.Moreover,compared with the group II and III,it was lower in coumarins like psoralen and isopsoralen.And the ingredients vary in the antidiarrhea efficacy:The flavonoid and volatile oil were highly correlated with the antidiarrhea efficacy,whereas the coumarin(such as psoralen and isopsoralen)had a low correlation.Conclusion After salt processing,Psoraleae Fructus and Foeniculif Fructus formed Buguzhi Pills,which can promote the dissolution of flavonoids and inhibit the dissolution of volatile oils and coumarins,this may be one of the key processes in salt processing to enhance the antidiarrhea effect.

关 键 词：补骨脂丸 补骨脂 小茴香 盐炙 配伍 止泻 HPLC-MS 脾肾阳虚 灰色关联分析 黄酮 香豆素 挥发油 

分 类 号：R283.1[医药卫生—中药学]