煅石膏中无水硫酸钙拉曼光谱定量分析方法研究  

Study on quantitative analysis method of anhydrous calcium sulfate in calcined gypsum by Raman spectroscopy

作　　者：彭艳[1] 张丽颖[1] 金阳[1] 杨钊 陆兔林[3] 金传山 都述虎[1] PENG Yan;ZHANG Li-ying;JIN Yang;YANG Zhao;LU Tu-lin;JIN Chuan-shan;DU Shu-hu(School of Pharmacy,Nanjing Medical University,Nanjing 211166,China;Joinative Traditional Chinese Medicine Industry Co.,Ltd,Wuhan 430040,China;School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210046,China;School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China)

机构地区：[1]南京医科大学药学院,江苏南京211166 [2]九州天润中药产业有限公司,湖北武汉430040 [3]南京中医药大学药学院,江苏南京210046 [4]安徽中医药大学药学院,安徽合肥230012

出　　处：《中草药》2021年第21期6683-6686,共4页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划资助(2018YFC1707000)。

摘　　要：目的建立拉曼光谱内标法快速测定煅石膏中无水硫酸钙的含量。方法内标物为硝酸钠,波长为780 nm,激光功率为24 mW,光阑为50μm slit,物镜为10倍,曝光时间为2 s,累积次数为2次,扫描范围为300~1700 cm^(−1)。结果无水硫酸钙质量分数在5%~35%与无水硫酸钙和硝酸钠的拉曼特征峰强度比(I1016/I1068)线性关系良好;低、中、高3个质量分数的平均加样回收率分别为101.4%、100.9%、100.6%,RSD分别为1.7%、0.8%、0.9%。结论该方法简便、快速、重复性好,可用于煅石膏药材的质量评价。Objective To establish an internal standard method for rapid determination of anhydrous calcium sulfate in calcined gypsum by Raman spectroscopy.Method Internal standard:sodium nitrate;excitation wavelength:780 nm;laser power:24 mW;aperture:50μm slit;lens:10×objective;exposure time:2 s;accumulation:2 times;scanning range:300-1700 cm^(−1).Results The peak intensity ratio(I1016/I1068)was used for the quantitative evaluation of calcined gypsum level and showed a good linear relationship with mass fraction of anhydrous calcium sulfate in the range of 5%-35%.The average recoveries at low,medium and high concentrations were 101.4%,100.9%and 100.6%,with RSD of 1.7%,0.8%and 0.9%,respectively.Conclusion The method is simple,rapid and reproducible.It can be used for the quality evaluation of calcined gypsum.

关 键 词：煅石膏 拉曼光谱 内标 无水硫酸钙 硝酸钠 

分 类 号：R286[医药卫生—中药学]