Chidamide诱导的LncRNA ENST00000448869.1靶向作用Nestin抑制乳腺癌MDA-MB-231细胞的生长  

Chidamide-induced LncRNA ENST00000448869.1 targeting Nestin inhibits growth of breast cancer MDA-MB-231 cells

作　　者：郭亚瑞 周伟强 GUO Ya-rui;ZHOU Wei-qiang(Dept of Pathogen Biology,School of Basic Medicine, Shenyang Medical College, Shenyang 110034, China)

机构地区：[1]沈阳医学院基础医学院病原生物学教研室,辽宁沈阳110034

出　　处：《中国药理学通报》2021年第12期1694-1699,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81172509);辽宁省教育厅科技项目(No SYYX202004);沈阳市科技局科技项目(No 20-205-4-066)。

摘　　要：目的研究在Chidamide抑制乳腺癌MDA-MB-231细胞生长中,LncRNA ENST00000448869.1靶向调控Nestin对细胞生长的影响。方法以人乳腺癌MDA-MB-231细胞为研究对象,通过高通量测序进行RNA文库筛查及测序比对分析,筛选经Chidamide处理后存在差异表达的LncRNA;利用蛋白互作生物学软件推测与该LncRNA互作的蛋白质;Muse细胞分析仪检测Chidamide对细胞活力的影响;Real-time PCR和Western blot检测Chidamide作用细胞后LncRNA及其互作蛋白的mRNA和蛋白表达的变化;特异siRNA敲除LncRNA后,Muse细胞分析仪检测细胞活力;Real-time PCR和Western blot检测LncRNA及其互作蛋白的mRNA和蛋白表达变化。结果从LncRNA大数据库筛查出ENST00000448869.1分子经Chidamide处理存在差异表达。蛋白互作生物学软件推测ENST00000448869.1分子与Nestin存在蛋白互作关系。Chidamide作用乳腺癌MDA-MB-231细胞后,抑制其生长,同时LncRNA ENST00000448869.1与Nestin的表达都增高;LncRNA-siRNA转染细胞后,细胞活力明显降低,LncRNA ENST00000448869.1和Nestin的表达都降低。结论在Chidamide处理MDA-MB-231细胞中,LncRNA ENST00000448869.1靶向调控Nestin抑制乳腺癌细胞生长。Aim To study the effect of targeted regulation of Nestin by LncRNA ENST00000448869.1 on the growth of breast cancer MDA-MB-231 cells when Chidamide inhibited the growth of these cells.Methods The human breast cancer MDA-MB-231 cells were taken as the research objects,and the LncRNA with differential expression after being treated with Chidamide was screened through RNA library screening and sequencing comparison analysis performed by high-throughput sequencing;using protein interaction biology software,the protein of interaction with LncRNA was estimated;the effect of chidamide on cell viability was detected by Muse cell analyzer.The mRNA and protein expression changes of LncRNA and its interaction protein after Chidamide action on cells were detected by Real-time PCR and Western blot.After LncRNA was knocked out by the specific siRNA,the cell viability was detected by Muse cell analyzer,the mRNA and protein expression levels of LncRNA and its interacting proteins were detected by Real-time PCR and Western blot.Results The differential expression of ENST00000448869.1 upon Chidamide treatment was screened from the large LncRNA database.Protein interaction biology software speculated that there was a protein interaction relationship between ENST00000448869.1 and Nestin.Chidamide inhibited the growth of breast cancer MDA-MB-231 cells and increased the expression of both LncRNA ENST00000448869.1 and Nestin.After the LncRNA-siRNA transfected cells,cell viability decreased significantly,and the expression levels of both LncRNA and Nestin decreased.Conclusions LncRNA ENST00000448869.1 targets regulation of Nestin to inhibit breast cancer cell growth in Chidamide-treated breast cancer MDA-MB-231 cells.

关 键 词：乳腺癌 MDA-MB-231 LncRNA ENST00000448869.1 CHIDAMIDE NESTIN 

分 类 号：R341[医药卫生—基础医学] R342.2