进境德国云杉原木中夹带栎树猝死病菌的检疫鉴定  

Quarantine identification of Phytophthora ramorum carried on Picea asperata imported from Germany

作　　者：李雪莲 吕燕 刘芳 张慧丽 段维军 LI Xuelian;Lü Yan;LIU Fang;ZHANG Huili;DUAN Weijun(Ningbo Academy of Inspection and Quarantine,Ningbo,Zhejiang 315012,China;Ningbo Custom District,Ningbo,Zhejiang 315012,China;Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]宁波检验检疫科学技术研究院,浙江宁波315012 [2]宁波海关,浙江宁波315012 [3]中国科学院微生物研究所,北京100101

出　　处：《生物安全学报》2021年第4期244-249,共6页Journal of biosafety

基　　金：国家重点研发计划(2016YFF0203201);国家自然科学基金(NSFC31770009);浙江省公益技术研究项目(LGF20C140001)。

摘　　要：【目的】检测自德国进境云杉原木夹杂的水青冈植物叶片上是否携带栎树猝死病菌。【方法】采用磁珠法提取水青冈植物叶片DNA,根据巢式PCR方法和DNA序列测定分析方法,进行栎树猝死病菌的检测。【结果】采用巢式PCR方法,能够扩增出约280 bp的特异性条带;DNA序列测定表明,该DNA序列与GenBank中多个栎树猝死病菌分离物DNA序列相似性达99%,位于同一个发育分支。【结论】样品中检出栎树猝死病菌,这是我国口岸首次从进境德国云杉原木中截获栎树猝死病菌。【Aim】To detect the Phytophthora ramorum on Picea asperata imported from Germany.【Method】Plant DNA was extracted by magnetic bead method.The nested PCR and DNA sequence analysis were carried out to detect the pathogen.【Result】Positive fragments were successfully amplified using specific nested PCR tests.The sequence obtained showed 99%identity to these sequences of P.ramorum deposited in GenBank.Phylogenetic analysis showed that the sequence was clustered in the same clade as P.ramorum.【Conclusion】To our knowledge,this is the first report of P.ramorum carried by P.asperata imported from Germany.

关 键 词：栎树猝死病菌 外来入侵物种 系统发育分析 检疫鉴定 

分 类 号：S763[农业科学—森林保护学]