Fluorescence recovery after photobleaching:analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery  

作　　者：Nan Zhang Kang Li Bin-Bin Xie Xiu-Lan Chen Bai-Cheng Zhou Hai-Nan Su Yu-Zhong Zhang 

机构地区：[1]State Key Laboratory of Microbial Technology,and Marine Biotechnology Research Center,Shandong University,Qingdao 266237,China [2]College of Marine Life Sciences,Frontiers Science Center for Deep Ocean Multispheres and Earth System,Ocean University of China,Qingdao 266003,China [3]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology,Qingdao 266237,China [4]College of Bioengineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250353,China

出　　处：《Marine Life Science & Technology》2021年第4期427-433,共7页海洋生命科学与技术（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(no.31900023);National Key R&D Program of China(no.2018YFC1406701);Program of Shandong Taishan Scholars(no.tspd20181203);Natural Science Foundation of Shandong(no.ZR2017LD013);AoShan Talents Cultivation Program(no.2017ASTCP-OS14);State Key Laboratory of Microbial Technology Open Projects Fund(no.M2019-07);Young Scholars Program of Shandong University(no.2017WLJH22).

摘　　要：Fluorescence recovery after photobleaching(FRAP)has been used to study the dynamics of the cyanobacterial photosynthesis apparatus since 1997.Fluorescence recovery of cyanobacteria during FRAP was conventionally interpreted as a result of phycobilisome(PBS)diffusion on the surface of the thylakoid membrane.The mechanism of state transition in cyanobacteria has been widely attributed to PBS diffusion.However,in red algae,another PBS-containing group,the intrinsic photoprocess was found to contribute greatly to the fluorescence recovery of PBS,which raises questions concerning the role of FRAP in red algal PBS.Therefore,it is important to re-evaluate the nature of PBS fluorescence recovery in cyanobacteria.In the present study,four cyanobacterial strains with different phenotypes and PBS compositions were used to investigate their FRAP characteristics.Fluorescence recovery of PBS was observed in wholly photobleached cells in all four cyanobacterial strains,in which the contribution of PBS diffusion to the fluorescence recovery was not possible.Moreover,the fluorescence recovered in isolated PBSs and PBS-thylakoid membranes after photobleaching further demonstrated the intrinsic photoprocess nature of fluorescence recovery.These findings suggest that the intrinsic photoprocess contributed to the fluorescence recovery following photobleaching when measured by the FRAP method.

关 键 词：CYANOBACTERIA Fluorescence recovery after photobleaching Intrinsic photoprocess Mobility PHYCOBILISOME 

分 类 号：Q94[生物学—植物学]